Volumetric Deformation of Live Cells Induced by Pressure-Activated Cross-Membrane Ion Transport
Volumetric Deformation of Live Cells Induced by Pressure-Activated Cross-Membrane Ion Transport
复制标题
压力激活跨膜离子传输引起的活细胞体积变形
DOI：
10.1103/physrevlett.113.118101
复制
发表时间：
2014-09-09
影响因子：
8.6
通讯作者：
Gao, H.
中科院分区：
文献类型：
--
作者：
Hui, T. H.;Zhou, Z. L.;Gao, H.
在这项工作中，我们开发了一种方法，允许通过介质中的静水压力变化精确控制细胞大小的变化。具体来说，我们表明，突然增加，或减少，在周围的压力，在生理相关的范围内，触发跨膜流量的钠和钾离子在白血病细胞系K562和HL 60，导致可逆的体积变形的特征时间约为30分钟。有趣的是，健康的白细胞不响应压力冲击，这表明癌细胞可能已经进化出适应微环境压力变化的能力。还提出了一个模型来解释所观察到的细胞变形，这突出了细胞的表观粘弹性响应是如何由微观跨膜运输。
In this work, we developed a method that allows precise control over changes in the size of a cell via hydrostatic pressure changes in the medium. Specifically, we show that a sudden increase, or reduction, in the surrounding pressure, in the physiologically relevant range, triggers cross-membrane fluxes of sodium and potassium ions in leukemia cell lines K562 and HL60, resulting in reversible volumetric deformation with a characteristic time of around 30 min. Interestingly, healthy leukocytes do not respond to pressure shocks, suggesting that the cancer cells may have evolved the ability to adapt to pressure changes in their microenvironment. A model is also proposed to explain the observed cell deformation, which highlights how the apparent viscoelastic response of cells is governed by the microscopic cross-membrane transport.