Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
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DOI：
10.3109/17435390.2010.536615
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发表时间：
2011-12-01
期刊：
影响因子：
5
通讯作者：
Elliott, John T.
中科院分区：
文献类型：
--
作者：
Zook, Justin M.;MacCuspie, Robert I.;Elliott, John T.
为了研究纳米颗粒在相关条件下的毒性，关键是将纳米颗粒以不同的团聚状态可重复地分散在与基于细胞的测定相容的水溶液中。在这里，我们分散金，银，氧化铈，和带正电的聚苯乙烯纳米粒子在细胞培养基中，使用混合步骤之间的时间来控制团聚体的大小在其他相同的介质。这些蛋白质稳定的分散体通常稳定至少两天，与23 nm银纳米颗粒相似的平均团聚体尺寸在43-1400 nm范围内，平均相对标准偏差小于10%。混合速率，混合步骤和纳米颗粒浓度之间的时间被证明是实现可再现的分散体的关键。我们进一步发展了动态光散射理论和扩散限制的胶体聚集理论来表征团聚纳米粒子的粒径分布。这些理论经常影响估计的大小的两个或更多的因素。最后，我们证明了控制团聚的重要性，显示大团聚体的银纳米粒子引起的溶血毒性显着低于小团聚体。
To study the toxicity of nanoparticles under relevant conditions, it is critical to disperse nanoparticles reproducibly in different agglomeration states in aqueous solutions compatible with cell-based assays. Here, we disperse gold, silver, cerium oxide, and positively-charged polystyrene nanoparticles in cell culture media, using the timing between mixing steps to control agglomerate size in otherwise identical media. These protein-stabilized dispersions are generally stable for at least two days, with mean agglomerate sizes of similar to 23 nm silver nanoparticles ranging from 43-1400 nm and average relative standard deviations of less than 10%. Mixing rate, timing between mixing steps and nanoparticle concentration are shown to be critical for achieving reproducible dispersions. We characterize the size distributions of agglomerated nanoparticles by further developing dynamic light scattering theory and diffusion limited colloidal aggregation theory. These theories frequently affect the estimated size by a factor of two or more. Finally, we demonstrate the importance of controlling agglomeration by showing that large agglomerates of silver nanoparticles cause significantly less hemolytic toxicity than small agglomerates.