S-Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the Golgi.
S-Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the Golgi.
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DOI：
10.1016/j.devcel.2018.10.024
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发表时间：
2018-11-19
影响因子：
11.8
通讯作者：
Rothman JE
中科院分区：
文献类型：
--
作者：
Ernst AM;Syed SA;Zaki O;Bottanelli F;Zheng H;Hacke M;Xi Z;Rivera-Molina F;Graham M;Rebane AA;Björkholm P;Baddeley D;Toomre D;Pincet F;Rothman JE
虽然已知高尔基群岛的逆行货物选择取决于特定的信号，但尚不清楚顺行货物是否被分类，以及顺行信号是否被识别。我们认为，高尔基体膜界面上顺行的S棕榈酰化是一种顺行信号，它通过简单的物理化学作用导致高尔基体边缘弯曲区域的聚集。两种S棕榈酰化的膜蛋白跨高尔基体转运的速率受Spalmitoyl化的控制。基于点击化学的荧光和电子显微镜显示，高尔基体中的大部分S棕榈酰化蛋白的行为类似。这些棕榈酰化的物质集中在高尔基体膜最弯曲的区域，包括脑池和相关囊泡的开窗周长。棕榈酰化的跨膜结构域在模型系统中的行为类似。存在用于逆行货物的分泌途径受体介导的蛋白质分选的例子，但不适用于顺行货物。恩斯特等人。揭示顺行的货物分选机制：高尔基体上的Spalmito化起到生物物理开关的作用，诱导膜上货物到池缘的“自我分选”，从而使其能够通过高尔基体高效地运输。
While retrograde cargo selection in the Golgi is known to depend on specific signals, it is unknown whether anterograde cargo is sorted and anterograde signals have not been identified. We suggest here that S-palmitoylation of anterograde cargo at the Golgi membrane interface is an anterograde signal, and that it results in concentration in curved regions at the Golgi rims by simple physical chemistry. The rate of transport across the Golgi of two S-palmitoylated membrane proteins is controlled by Spalmitoylation. The bulk of S-palmitoylated proteins in the Golgi behave analogously, as revealed by click chemistry-based fluorescence and electron microscopy. These palmitoylated cargo concentrate in the most highly curved regions of the Golgi membranes, including the fenestrated perimeters of cisternae and associated vesicles. A palmitoylated transmembrane domain behaves similarly in model systems. Examples exist of secretory pathway receptor-mediated protein sorting for retrograde cargo but not for anterograde. Ernst et al. uncover an anterograde cargo sorting mechanism: Spalmitoylation at the Golgi acts as a biophysical switch that induces “self-sorting” of membrane cargo to the cisternal rim, enabling its efficient transport through the Golgi.
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DOI：
10.1074/jbc.m802140200
发表时间：
2008-09-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Greaves J;Salaun C;Fukata Y;Fukata M;Chamberlain LH
通讯作者：
Chamberlain LH
影响因子：
4.8
作者：
Gottlieb, Colin D.;Zhang, Sheng;Linder, Maurine E.
通讯作者：
Linder, Maurine E.
影响因子：
5.3
作者：
Apolloni, A;Prior, IA;Hancock, JF
通讯作者：
Hancock, JF
影响因子：
4.8
作者：
Lobo, S;Greentree, WK;Deschenes, RJ
通讯作者：
Deschenes, RJ
影响因子：
4.8
作者：
Du, Keyong;Murakami, Shoko;Luscher, Bernhard
通讯作者：
Luscher, Bernhard