ESTIMATION OF 2 COLLAGENS FROM HUMAN DERMIS BY INTERRUPTED GEL-ELECTROPHORESIS
ESTIMATION OF 2 COLLAGENS FROM HUMAN DERMIS BY INTERRUPTED GEL-ELECTROPHORESIS
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DOI：
10.1016/s0006-291x(76)80180-5
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发表时间：
1976-01-01
影响因子：
3.1
通讯作者：
SMITH, R
中科院分区：
文献类型：
--
作者：
SYKES, B;PUDDLE, B;SMITH, R
描述了2种人软组织胶原[α 1（1）] 2 α 2和[α 1（111）]3在聚丙烯酰胺凝胶电泳上的比较行为。延迟二硫键连接的[α 1（111）]3的还原直到电泳开始后1小时，导致所有3种物种的良好分离。的α-链存在于混合物中。这种分离允许定量染色的凝胶密度;这是如何应用于快速估计的相对比例的2种胶原蛋白的范围内的人类皮肤样本进行描述。
The comparative behavior of 2 human soft tissue collagens [.alpha.1 (1)]2.alpha.2, and [.alpha.1 (111)]3 on polyacrylamide gel electrophoresis is described. Delay in the reduction of disulfide-linked [.alpha.1 (111]3 until 1 h after electrophoresis has begun results in a good separation of all 3 spp. of .alpha.-chain present in a mixture. This separation allows quantitation of stained gels by densitometry; how this was applied to the rapid estimation of the relative proportions of the 2 collagens in a range of human skin samples is described.