MS-2 AND POLIOVIRUS TRANSPORT IN POROUS-MEDIA - HYDROPHOBIC EFFECTS AND CHEMICAL PERTURBATIONS
MS-2 AND POLIOVIRUS TRANSPORT IN POROUS-MEDIA - HYDROPHOBIC EFFECTS AND CHEMICAL PERTURBATIONS
复制标题
DOI：
10.1029/92wr02986
复制
发表时间：
1993-04-01
影响因子：
5.4
通讯作者：
GERBA, CP
中科院分区：
文献类型：
--
作者：
BALES, RC;LI, SM;GERBA, CP
在一系列的pH 7连续流柱实验中，通过附着到二氧化硅珠上去除噬菌体MS-2对珠上存在的疏水表面的量具有强烈的系统依赖性。由于没有疏水表面，在pH 5下去除噬菌体比在pH 7下大得多。在两种pH值下都发生了附着的噬菌体的释放，但缓慢;穿透曲线显示拖尾。脊髓灰质炎病毒在pH 5.5莫尔比在pH 7.0时更多地附着在二氧化硅珠上，并且附着也是缓慢可逆的。噬菌体和脊髓灰质炎病毒附着的时间尺度为小时量级。粘附效率因子（α）反映了病毒附着的微尺度理化影响，其范围为0.0007-0.02。噬菌体释放量很小，但在稳态条件下可测量。通过降低离子强度和引入牛肉提取物（一种高离子强度蛋白质溶液）来增强释放。结果表明，病毒经历可逆的附着/脱离（有时称为吸附），需要大的化学扰动诱导病毒快速脱离，病毒应该是相当移动的在桑迪多孔介质。即使多孔介质中少量的疏水有机材料（大于或等于0.001%）也会阻碍病毒转运。
In a series of pH 7 continuous-flow column experiments, removal of the bacteriophage MS-2 by attachment to silica beads had a strong, systematic dependence on the amount of hydrophobic surface present on the beads. With no hydrophobic surface, removal of phage at p H 5 was much greater than at pH 7. Release of attached phage at both pH values did occur, but was slow; breakthrough curves exhibited tailing. Poliovirus attached to silica beads at pH 5.5 much mor, than at pH 7.0, and attachment was also slowly reversible. Time scales for phage and poliovirus attachment were of the order of hours. The sticking efficiency factor (alpha), reflecting microscale physicochemical influences on virus attachment, was in the range of 0.0007-0.02. Phage release was small but measurable under steady state conditions. Release was enhanced by lowering ionic strength and by introducing beef extract, a high-ionic-strength protein solution. Results show that viruses experience reversible attachment/detachment (sometimes termed sorption), that large chemical perturbations are needed to induce rapid virus detachment, and that viruses should be quite mobile in sandy porous media. Even small amounts of hydrophobic organic material in the porous media (greater-than-or-equal-to 0.001%) can retard virus transport.