Nanofibers from Blends of Polyvinyl Alcohol and Polyhydroxy Butyrate As Potential Scaffold Material for Tissue Engineering of Skin
Nanofibers from Blends of Polyvinyl Alcohol and Polyhydroxy Butyrate As Potential Scaffold Material for Tissue Engineering of Skin
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DOI：
10.1021/bm100912v
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发表时间：
2010-12-01
影响因子：
6.2
通讯作者：
Groth, T.
中科院分区：
文献类型：
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作者：
Asran, Ashraf Sh.;Razghandi, Kashayar;Groth, T.
以聚乙烯醇（PVA）、聚羟基丁酸酯（PHB）及其共混物为原料，采用静电纺丝法制备纳米纤维。采用傅里叶变换红外光谱（FTIR）、扫描电镜（SEM）、透射电镜（TEM）和差示扫描量热仪（DSC）研究了两种聚合物在共混体系中的相容性和形态结构。DSC也显示出随着PVA比例的增加，共混纳米纤维中PHB的结晶度受到抑制。随着PVA含量的增加，PHB的水解降解加快。细胞培养实验与人角质形成细胞系（HaCaT）和真皮成纤维细胞上的静电纺丝的聚羟基丁酸和PVA/聚羟基丁酸共混物纳米纤维显示最大的粘附和增殖纯聚羟基丁酸。然而，添加5重量%的聚乙烯醇，以聚羟基丁酸抑制HaCaT细胞的生长，但不是成纤维细胞。相反，HaCaT细胞在PVA/PHB（50/50）纤维上的粘附和增殖受到促进，而成纤维细胞的生长受到抑制。
Nanofibers were prepared by electrospinning from pure polyvinyl alcohol (PVA), polyhydroxybutyrate (PHB), and their blends. Miscibility and morphology of both polymers in the nanofiber blends were studied by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and differential scanning calorimetry (DSC), revealing that PVA and PHB were miscible with good compatibility. DSC also revealed suppression of crystallinity of PHB in the blend nanofibers with increasing proportion of PVA. The hydrolytic degradation of PHB was accelerated with increasing PVA fraction. Cell culture experiments with a human keratinocyte cell line (HaCaT) and dermal fibroblast on the electrospun PHB and PVA/PHB blend nanofibers showed maximum adhesion and proliferation on pure PHB. However, the addition of 5 wt % PVA to PHB inhibited growth of HaCaT cells but not of fibroblasts. On the contrary, adhesion and proliferation of HaCaT cells were promoted on PVA/PHB (50/50) fibers, which inhibited growth of fibroblasts.