Molecular epidemiology of rabies in Guangxi Province, south of China
Molecular epidemiology of rabies in Guangxi Province, south of China
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DOI：
10.1016/j.jcv.2007.04.021
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发表时间：
2007-08-01
影响因子：
8.8
通讯作者：
Li, Hua-Ming
中科院分区：
文献类型：
--
作者：
Liu, Qi;Xiong, Yi;Li, Hua-Ming
背景：中国广西省狂犬病监测数据显示，自1996年以来，人类狂犬病病例逐渐增加。目的：从分子水平评价广西狂犬病的流行病学，为有效预防狂犬病提供建议。Studv设计。-自2000年以来。在广西不同地区采集疑似狂犬病动物脑1569个。从42份样本中分离出狂犬病毒。RT-PCR扩增了N基因y端455核位片段。该片段的测序数据用于系统发育分析。结果：基于该序列的核苷酸同源性比较和系统进化树分析表明，广西所有狂犬病毒分离株均为基因型I，可分为4个类群。1组、11组和IV组分别有23株、10株和8株。核苷酸同源性分别为97.1-100%、99.2 -100%和99.1-99.6%。只有gxn119菌株属于111群。组1有两个组特异性突变：T90N和El 101)。第11组有1个T42S组特异性突变。结论：广西狂犬病病毒分离株具有密切的亲缘关系和地理分布。(c) 2007 Elsevier B.V.版权所有
Background: Surveillance data for rabies in Guangxi Province in China showed that human rabies cases have gradually increased since 1996.Objective: To evaluate the epidemiology of rabies at the molecular level and provide suggestions for effective prevention of rabies in Guangxi.Studv design.- Since 2000. 1569 brains from suspected rabid animals were collected from different areas of Guangxi. Rabies virus was isolated from 42 samples. RT-PCR was used to amplify a 455 nucleoticle segment of the Y-terminal of the N gene. The sequencing data from that segment was used for phylogenetic analysis.Results: Nucleotide homology comparisons and phylogenetic tree analysis based on this sequence indicated that all the rabies virus isolates from Guangxi belonged to genotype I and could be divided into four groups. Groups 1, 11 and IV included 23, 10 and 8 isolates, respectively. These had nucleotide homologies of 97.1-100%, 98.2-100% and 99.1-99.6%, respectively. Only the GXN 119 strain belonged to group 111. Group I had two group-specific mutations: T90N and El 101). Group 11 had one group-specific mutation of T42S.Conclusions: This study showed that rabies virus isolates from Guangxi have a close genetic relationship and topographical distribution. (c) 2007 Elsevier B.V. All rights reserved.