Improving Survival of Disassociated Human Embryonic Stem Cells by Mechanical Stimulation Using Acoustic Tweezing Cytometry
Improving Survival of Disassociated Human Embryonic Stem Cells by Mechanical Stimulation Using Acoustic Tweezing Cytometry
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DOI：
10.1016/j.bpj.2015.01.033
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发表时间：
2015-03-24
影响因子：
3.4
通讯作者：
Fu, Jianping
中科院分区：
文献类型：
--
作者：
Chen, Di;Sun, Yubing;Fu, Jianping
解离诱导的人胚胎干细胞(HESCs)的凋亡阻碍了其大规模培养。在这里，我们利用人胚胎干细胞的机械敏感性，据我们所知，使用了一种新的技术--声镊式细胞术(ATC)来对分离的单个人胚胎干细胞进行亚细胞机械刺激，以提高其存活率。通过声学作用于活细胞的整合素结合微泡(MBS)，ATC将hESCs的存活率和克隆效率提高了三倍。在ATC刺激过程中，hESC存活率的增加与整合素锚定的MBS的总累积位移呈正相关。ATC可以作为一种有前景的生物相容性工具来改进hESC的培养。
Dissociation-induced apoptosis of human embryonic stem cells (hESCs) hampers their large-scale culture. Herein we leveraged the mechanosensitivity of hESCs and employed, to our knowledge, a novel technique, acoustic tweezing cytometry (ATC), for subcellular mechanical stimulation of disassociated single hESCs to improve their survival. By acoustically actuating integrin-bound microbubbles (MBs) to live cells, ATC increased the survival rate and cloning efficiency of hESCs by threefold. A positive correlation was observed between the increased hESC survival rate and total accumulative displacement of integrin-anchored MBs during ATC stimulation. ATC may serve as a promising biocompatible tool to improve hESC culture.