Bioassay for determination of fosmidomycin in plasma and urine: Application for pharmacokinetic dose optimisation
Bioassay for determination of fosmidomycin in plasma and urine: Application for pharmacokinetic dose optimisation
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DOI：
10.1016/j.mimet.2006.11.018
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Na-Bangchang, Kesara
中科院分区：
文献类型：
--
作者：
Cheoymang, Anurak;Hudchinton, David;Na-Bangchang, Kesara
建立了琼脂扩散盘法测定人血浆和尿液中fosmidomycin和clindamycin的简便、灵敏、选择性好、重复性好的方法。使用磁盘扩散技术，基本上与前面描述的一样，但利用试验生物阴沟肠杆菌ATCC 23355菌株在琼脂试验板上播种。血浆（0、1、2.5、5、7.5、10、25、50 ng/mu l）和尿液（0、10、25、50、75、100、250、500 mu g/mu l）浓度响应曲线均呈线性关系，相关系数均大于0.990。基于日内重复性和再现性（日变化）的方法精密度低于5%（%变异系数：%C.V.）。在日间或日间的测定中都观察到良好的准确性，正如测量样品的平均值与理论值的最小偏差（低于5%）所表明的那样。血浆40 μ l或尿样7.5 μ l定量限（L.O.Q.）为1 ng。磷霉素的平均回收率大于99%。本方法不受克林霉素、卡比西林、头孢菌素、氯霉素、卡那霉素、甲氧西林、青霉素、红霉素、林可霉素、四环素、帕罗霉素等常用抗生素的干扰。该方法似乎是可靠的，并已应用于疟疾患者在每8小时口服1200mg克林霉素7天后血浆和尿中磷霉素排泄的药代动力学研究。(c) 2006 Elsevier B.V.版权所有
A simple, sensitive, selective and reproducible method based on agar diffusion disk assay was developed for the determination of fosmidomycin and clindamycin in human plasma and urine. A disk diffusion technique was used, essentially as previously described but utilising the assay organism Enterobacter cloacae ATCC 23355 strain to seed the agar assay plates. Calibration curves were prepared from concentration response curves in plasma (0, 1, 2.5, 5, 7.5, 10, 25, 50 ng/mu l) and urine (0, 10, 25, 50, 75, 100, 250 and 500 mu g/mu l) were all linear with correlation coefficients better than 0.990. The precision of the method based on within-day repeatability and reproducibility (day-to-day variation) was below 5% (% coefficient of variations: %C.V.). Good accuracy was observed for both the intra-day or inter-day assays, as indicated by the minimal deviation of mean values found with measured samples from that of the theoretical values (below 5%). Limit of quantification (L.O.Q.) was accepted as 1 ng using 40-mu l plasma or 7.5-mu l urine sample. The mean recovery for fosmidomycin was greater than 99%. The method was free from interference from other commonly used antibiotics including clindamycin, carbenicillin, cephalothin, chloramphenicol, kanamycin, methicillin, penicillin, erythromycin, lincomycin, tetracycline and paromomycin. The method appears to be robust and has been applied to a pharmacokinetic study in plasma and urinary excretion of fosmidomycin in a patient with malaria following oral doses of clindamycin at 1200 mg given every 8 h for 7 days. (c) 2006 Elsevier B.V. All rights reserved.