Hindsight/RREB-1 functions in both the specification and differentiation of stem cells in the adult midgut of Drosophila.
Hindsight/RREB-1 functions in both the specification and differentiation of stem cells in the adult midgut of Drosophila.
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DOI：
10.1242/bio.015636
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发表时间：
2015-12-10
期刊：
影响因子：
2.4
通讯作者：
Reed BH
中科院分区：
文献类型：
--
作者：
Baechler BL;McKnight C;Pruchnicki PC;Biro NA;Reed BH
果蝇成虫中肠是由未分化的中肠前体细胞（AMP）在幼虫/蛹转变过程中形成的。在成体中肠上皮中发现了四种基本细胞类型：未分化的肠干细胞（ISC）及其定向子细胞、肠母细胞（EB）、肠上皮细胞（EC）和肠内分泌细胞（EE）。以果蝇后中肠为模型，研究了转录因子Hindsight（Hnt）/RREB-1的功能及其与Notch和Egfr信号通路的关系。我们发现，hnt是EC分化所需的ISC-EC分化的背景下，但不是在AMP-EC分化的背景下。此外，我们表明，hnt是必要的建立可行的或功能的ISCs。总的来说，我们的研究将hnt作为一个关键因子引入到发育和成熟的成体中肠的调控中。我们认为，这些背景差异的性质可以通过hnt与多种信号通路的相互作用来解释。总结：RREB-1的果蝇同源物，后见之明，可以促进肠上皮细胞分化，特别是在成年中肠。幼虫/蛹转变期间的肠上皮细胞分化不需要后见之明。
The adult Drosophila midgut is established during the larval/pupal transition from undifferentiated cells known as adult midgut precursors (AMPs). Four fundamental cell types are found in the adult midgut epithelium: undifferentiated intestinal stem cells (ISCs) and their committed daughter cells, enteroblasts (EBs), plus enterocytes (ECs) and enteroendocrine cells (EEs). Using the Drosophila posterior midgut as a model, we have studied the function of the transcription factor Hindsight (Hnt)/RREB-1 and its relationship to the Notch and Egfr signaling pathways. We show that hnt is required for EC differentiation in the context of ISC-to-EC differentiation, but not in the context of AMP-to-EC differentiation. In addition, we show that hnt is required for the establishment of viable or functional ISCs. Overall, our studies introduce hnt as a key factor in the regulation of both the developing and the mature adult midgut. We suggest that the nature of these contextual differences can be explained through the interaction of hnt with multiple signaling pathways. Summary: Hindsight, the Drosophila homolog of RREB-1, can promote and is required for enterocyte differentiation specifically in the adult midgut. Enterocyte differentiation during the larval/pupal transition does not require Hindsight.