Hearing Loss in Experimental Cytomegalovirus Infection of the Guinea Pig Inner Ear: Prevention by Systemic Immunity
Hearing Loss in Experimental Cytomegalovirus Infection of the Guinea Pig Inner Ear: Prevention by Systemic Immunity
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豚鼠内耳实验性巨细胞病毒感染导致的听力损失：通过全身免疫预防
DOI：
10.1177/000348948509400405
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
D. Richman
中科院分区：
文献类型：
--
作者：
N. Woolf;J. Harris;D. Butler;A. Ryan;D. Richman
豚鼠巨细胞病毒（GPCMV）已被用于建立病毒性中耳炎和听力损失的可重复模型。通过耳蜗颤噪（CM）和第八神经N1复合动作电位（AP）阈值的电生理记录，在鼓阶接种前和接种后8天内评估耳蜗功能。内耳接种血清阴性受试者与活GPCMV产生深刻的海拔CM和AP阈值：70%的这些受试者的阈值提高到整个测试频率范围为0.10至32 kHz的声音系统的限制。与CM和AP阈值偏移相关的组织学效应主要局限于耳蜗周围室，并且在最基础的耳蜗转弯中最大。全身感染GPCMV产生免疫应答，但不影响CM或AP阈值。随后用活GPCMV接种这些血清学阳性动物的内耳未导致CM或AP阈值偏移或耳蜗组织病理学。将灭活病毒接种到血清阴性和血清阳性动物的内耳中仅产生中等CM和AP阈值效应。因此，原发性GPCMV耳蜗炎导致显著的耳蜗功能障碍和组织病理学变化，这可以通过先前全身感染GPCMV来预防。
Guinea pig cytomegalovirus (GPCMV) has been used to establish a reproducible model of viral labyrinthitis and hearing loss. Cochlear function was assessed by electrophysiological recordings of cochlear microphonic (CM) and eighth nerve N1 compound action potential (AP) thresholds prior to and up to eight days following inoculation of the scala tympani. Inner ear inoculation of seronegative subjects with live GPCMV produced profound elevations in CM and AP thresholds: 70% of these subjects had their thresholds raised to the limits of the sound system throughout the tested frequency range of 0.10 to 32 kHz. Histopathologic effects associated with CM and AP threshold shifts were primarily limited to the perilabyrinthine compartment, and were greatest in the most basal cochlear turns. Systemic infection with GPCMV produced an immune response, but did not affect CM or AP thresholds. Subsequent inoculation of the inner ear of these seropositive animals with live GPCMV did not result in either CM or AP threshold shifts, or cochlear histopathology. Inoculations of inactivated virus into the inner ears of seronegative and seropositive animals produced only moderate CM and AP threshold effects. Primary GPCMV labyrinthitis thus results in significant cochlear dysfunction and histopathologic changes which are prevented by prior systemic infection with GPCMV.