Hypocholesterolemic effect of stilbenes containing extract-fraction from Cajanus cajan L. on diet-induced hypercholesterolemia in mice
Hypocholesterolemic effect of stilbenes containing extract-fraction from Cajanus cajan L. on diet-induced hypercholesterolemia in mice
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含有木豆提取物的二苯乙烯对小鼠饮食诱导的高胆固醇血症的低胆固醇作用。
DOI：
10.1016/j.phymed.2008.03.002
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发表时间：
2008-11-01
期刊：
影响因子：
7.9
通讯作者：
Chen, Di-Hua
中科院分区：
文献类型：
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作者：
Luo, Qing-Feng;Sun, Lan;Chen, Di-Hua
Cajanus cajan (L) 是一种天然植物，含有大量潜在的活性成分。在本研究中，我们确定了含有二苯乙烯类木豆提取物（sECC）对昆明小鼠饮食诱导（4 周）高胆固醇血症的影响。所有实验小鼠均分为5组：对照组、模型组、sECC 200或100 mg/kg/天治疗组、辛伐他汀组。通过监测这些小鼠的血清和肝脏脂质谱（胆固醇稳态和甘油三酯）以及血清超氧化物歧化酶活性来研究 sECC 的影响。为了进一步探讨sECC的机制，通过逆转录PCR分析了肝脏3-羟基-3-甲基戊二酰辅酶A还原酶（HMG-CoA还原酶）、胆固醇7α-羟化酶（CYP7A1）和低密度脂蛋白受体（LDL受体）在胆固醇稳态中的表达。预处理4周后，与模型组相比，sECC（200mg/kg）升高的血清和肝脏总胆固醇显着减弱31.4%和22.7%（p
Cajanus cajan (L) is a natural plant which contains a lot of potential active components. In the present study, we identified the effects of the stilbenes containing extract-fraction from Cajanus cajan L (sECC) on diet-induced (for 4 weeks) hypercholesterolemia in Kunming mice. All experimental mice were divided into 5 groups: control group, model group, sECC-treated with 200 or 100 mg/kg/day, and simvastatin group. The effects of sECC were investigated by monitoring serum and liver lipid profile (cholesterol homeostasis and triglyceride) as well as serum superoxide dismutase activity in those mice. To further explore the mechanism of sECC, hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase), cholesterol 7 alpha-hydroxylase (CYP7A1), and low density lipoprotein receptor (LDL receptor) expressions in cholesterol homeostasis were analyzed by reverse transcription PCR. After 4 weeks pretreatment, compared with model group, the increased serum and hepatic total cholesterol were markedly attenuated by sECC (200mg/kg) by 31.4% and 22.7% (p