The phenotype and tissue-specific nature of multipotent cells derived from human mature adipocytes
The phenotype and tissue-specific nature of multipotent cells derived from human mature adipocytes
复制标题
人类成熟脂肪细胞衍生的多能细胞的表型和组织特异性
DOI：
10.1016/j.bbrc.2014.01.077
复制
发表时间：
2014-02-21
影响因子：
3.1
通讯作者：
Shen, Jie-Fei
中科院分区：
文献类型：
--
作者：
Kou, Liang;Lu, Xiao-Wen;Shen, Jie-Fei
成熟脂肪细胞来源的去分化脂肪细胞(DFAT)被认为是一组同质的多能细胞，有望成为再生医学成体干细胞的替代来源。然而，有关DFAT细胞的许多细胞性质仍不清楚。本研究旨在阐明DFAT细胞的基本特征及其功能和分化潜能。采用改良的顶盖培养技术，将人颊脂垫中的成熟脂肪细胞转化为DFAT细胞。流式细胞仪分析显示，这些来源的细胞是由CD13(+)、CD29(+)、CD105(+)、CD44(+)、CD31(-)、CD34(-)、CD309(-)α-SMA(-)细胞组成的均一群体。本研究中的DFAT细胞表现出组织特异性分化特性，成脂能力强，成骨能力弱得多。在血管生成诱导下，它们也不表达内皮标记物。(C)2014 Elsevier Inc.保留所有权利。
Dedifferentiated fat (DFAT) cells derived from mature adipocytes have been considered to be a homogeneous group of multipotent cells, which present to be an alternative source of adult stem cells for regenerative medicine. However, many aspects of the cellular nature about DFAT cells remained unclarified. This study aimed to elucidate the basic characteristics of DFAT cells underlying their functions and differentiation potentials. By modified ceiling culture technique, DFAT cells were converted from human mature adipocytes from the human buccal fat pads. Flow cytometry analysis revealed that those derived cells were a homogeneous population of CD13(+) CD29(+) CD105(+) CD44(+) CD31(-) CD34(-) CD309(-) alpha-SMA(-) cells. DFAT cells in this study demonstrated tissue-specific differentiation properties with strong adipogenic but much weaker osteogenic capacity. Neither did they express endothelial markers under angiogenic induction. (C) 2014 Elsevier Inc. All rights reserved.