Phylogeny, Pathogenicity, and Transmission of H5N1 Avian Influenza Viruses in Chickens.
Phylogeny, Pathogenicity, and Transmission of H5N1 Avian Influenza Viruses in Chickens.
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DOI：
10.3389/fcimb.2017.00328
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发表时间：
2017
影响因子：
5.7
通讯作者：
Liao M
中科院分区：
文献类型：
--
作者：
Cui J;Qu N;Guo Y;Cao L;Wu S;Mei K;Sun H;Lu Y;Qin Z;Jiao P;Liao M
我们分析了 2012 年从中国不同鸟类中分离出的 5 种 H5N1 禽流感病毒 (AIV)。根据全基因组序列，我们将病毒分为四种基因型。 DKE26、GSE43 和 DKE53 病毒分别属于基因型 1-3。 CKE93和CKE96病毒被分为基因型4。基因型1-3对应于含有进化枝2.3.2的HA基因的病毒，基因型4是携带进化枝7.2的HA基因的病毒。为了更好地了解病毒的致病性和传播，我们用 103 只 EID50/0.1 ml GSE43（进化枝 2.3.2）或 CKE93（进化枝 7.2）病毒感染鸡。我们的结果显示，接种 GSE43 病毒的 7 只无特定病原体 (SPF) 鸡中，有 6 只在感染后 7 天前死亡，但接种 CKE93 病毒的 SPF 鸡全部存活。 GSE43 和 CKE93 病毒都在鸡体内进行系统复制。受检器官中GSE43病毒的病毒滴度明显高于CKE93病毒。我们的结果表明，GSE43 在鸡中的致病性和复制性远高于 CKE93。 GSE43病毒可以在鸡之间传播，但CKE93病毒不能通过幼稚接触在鸡之间传播。因此，不同分支的H5N1禽流感病毒在鸡群中具有不同的致病性和传播能力。我们的研究有助于了解流行的 H5N1 病毒的致病变异。
We analyzed five H5N1 avian influenza viruses (AIVs) isolated from different birds in 2012 in China. Based on whole-genome sequences, we divided the viruses into four genotypes. The DKE26, GSE43, and DKE53 viruses belonged to Genotypes 1–3, respectively. The CKE93 and CKE96 viruses were classified into Genotype 4. Genotypes 1–3 correspond to the viruses containing the HA gene of clade 2.3.2, and Genotype 4 is the virus that bears the HA gene of clade 7.2. To better understand the pathogenicity and transmission of the viruses, we infected chickens with 103 EID50/0.1 ml GSE43 (clade 2.3.2) or CKE93 (clade 7.2) virus. Our results revealed that 6 of 7 specific-pathogen-free (SPF) chickens inoculated with GSE43 virus were dead before 7-day post-infection, but all the SPF chickens inoculated with CKE93 virus survived the infection. Both the GSE43 and CKE93 viruses replicated systemically in chickens. The virus titers of GSE43 virus in tested organs were obviously higher than those of CKE93 virus. Our results revealed that the pathogenicity and replication of GSE43 in chickens was much higher than those of CKE93. The GSE43 virus could transmit between chickens, but the CKE93 could not transmit between chickens by naïve contact. Therefore, different clades of H5N1 AIVs possessed variable pathogenicities and transmission abilities among chickens. Our study contributes to knowledge of pathogenic variations of prevalent H5N1 viruses.