Serotonin-immunoreactive sensory neurons in the antenna of the cockroach Periplaneta americana
Serotonin-immunoreactive sensory neurons in the antenna of the cockroach Periplaneta americana
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美洲大蠊蟑螂触角中的血清素免疫反应性感觉神经元
DOI：
10.1002/cne.23419
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发表时间：
2014
期刊：
影响因子：
2.5
通讯作者：
Yokohari F
中科院分区：
文献类型：
--
作者：
Watanabe H;Shimohigashi M;Yokohari F
昆虫的触角包含大量的感觉神经元，这些神经元处理嗅觉、味觉、机械感觉、湿感和温度感觉信息。除了具有多通道功能的感觉神经元外，大多数感觉神经元都使用乙酰胆碱作为神经递质。用免疫组织化学方法结合美洲大蠊触角感觉神经元的逆行染色，我们在触角内发现了5-羟色胺免疫反应的感觉神经元。它们选择性地分布在节节和节节感受器以及鞭毛的花序、花梗和前15节。在杂乱的感受器中，一个5-羟色胺免疫反应阳性的感觉神经元与多达4个5-羟色胺免疫反应阴性的感觉神经元共存。根据其形态特征，5-羟色胺免疫阳性感觉神经元和阴性感觉神经元可能分别进行机械感觉和接触化学感觉。棘突感受器构成椎弓根内的脊索器和约翰斯顿器官，处理触角的振动。免疫电子显微镜清楚地显示，5-羟色胺免疫反应选择性地定位于一种特定类型的机械感觉神经元，称为1型感觉神经元。在脊索节节突感受器中，5-羟色胺免疫反应阳性的1型神经元总是与5-羟色胺阴性的1型神经元配对。相反，5-羟色胺免疫阳性和阴性的1型神经元在Johnston‘s器官中随机分布。在中脑，5-羟色胺免疫反应的感觉神经元轴突形成三种不同的感觉束，来自不同类型感受器的轴突终止于不同的大脑区域。我们的发现表明，一种生物胺，5-羟色胺，可能在外周机械感觉神经元中扮演神经递质的角色。J.Comp.神经。2014年522：414-434。©2013 Wiley期刊，Inc.
The antennae of insects contain a vast array of sensory neurons that process olfactory, gustatory, mechanosensory, hygrosensory, and thermosensory information. Except those with multimodal functions, most sensory neurons use acetylcholine as a neurotransmitter. Using immunohistochemistry combined with retrograde staining of antennal sensory neurons in the cockroachPeriplaneta americana, we found serotonin‐immunoreactive sensory neurons in the antenna. These were selectively distributed in chaetic and scolopidial sensilla and in the scape, the pedicel, and first 15 segments of the flagellum. In a chaetic sensillum, A single serotonin‐immunoreactive sensory neuron cohabited with up to four serotonin‐negative sensory neurons. Based on their morphological features, serotonin‐immunopositive and ‐negative sensory neurons might process mechanosensory and contact chemosensory modalities, respectively. Scolopidial sensilla constitute the chordotonal and Johnston's organs within the pedicel and process antennal vibrations. Immunoelectron microscopy clearly revealed that serotonin‐immunoreactivities selectively localize to a specific type of mechanosensory neuron, called type 1 sensory neuron. In a chordotonal scolopidial sensillum, a serotonin‐immunoreactive type 1 neuron always paired with a serotonin‐negative type 1 neuron. Conversely, serotonin‐immunopositive and ‐negative type 1 neurons were randomly distributed in Johnston's organ. In the deutocerebrum, serotonin‐immunoreactive sensory neuron axons formed three different sensory tracts and those from distinct types of sensilla terminated in distinct brain regions. Our findings indicate that a biogenic amine, serotonin, may act as a neurotransmitter in peripheral mechanosensory neurons. J. Comp. Neurol. 522:414–434, 2014. © 2013 Wiley Periodicals, Inc.