A versatile luminescent resonance energy transfer (LRET)-based ratiometric upconversion nanoprobe for intracellular miRNA biosensing
A versatile luminescent resonance energy transfer (LRET)-based ratiometric upconversion nanoprobe for intracellular miRNA biosensing
复制标题
用于细胞内 miRNA 生物传感的多功能发光共振能量转移 (LRET) 比例上转换纳米探针
DOI：
10.1039/d0tb00820f
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发表时间：
2020-07-21
影响因子：
7
通讯作者：
Xu, Lijian
中科院分区：
文献类型：
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作者：
Gong, Liang;Liu, Simin;Xu, Lijian
合理设计和制造用于细胞内miRNA生物传感的生物纳米探针对早期临床诊断和预后具有重要意义。在此，我们开发了一种多功能的基于lret的比率（LBRU）纳米探针，该纳米探针由NaYF4:Yb，Er@NaYF4@NH2-mSiO(2)/罗丹明B/C-DNA三明治结构纳米复合材料组成，用于细胞内miRNA生物传感。纳米探针由NaYF4:Yb、Er@NaYF(4)上转化纳米粒子（能量供体）和氨基功能化介孔二氧化硅壳（NH2-mSiO(2)）、罗丹明B（受体）负载在NH2-mSiO(2)的介孔中以及靶miRNA（标记为C-DNA）的互补序列作为识别物质包裹在纳米复合材料上组成。由于供体和受体之间的LRET行为，负载的罗丹明B可以完全猝灭NaYF4:Yb，Er@NaYF(4)在540 nm处的绿色上转换发射。此外，它可以在目标miRNA存在的情况下从纳米复合材料中释放出来，从而阻止LRET行为“打开”绿色上转换发光。此外，由于未受影响的红色上转换发光（660nm）可作为内标，对环境效应进行内置校正，因此采用540和660 nm上转换发光的强度比（I-540/I-660）作为输出信号，以准确检测目标miRNA。由于该纳米探针具有生物相容性、高光稳定性和低自荧光背景，因此成功地利用转换荧光成像技术诊断了MCF-7细胞内miRNA-21的表达。我们设想LBRU纳米探针在早期癌症诊断中具有很大的应用潜力。
Rational design and fabrication of bio-nanoprobes for intracellular miRNA biosensing are highly desired for early clinical diagnosis and prognosis. Herein, we have developed a versatile LRET-based ratiometric (LBRU) nanoprobe of NaYF4:Yb,Er@NaYF4@NH2-mSiO(2)/rhodamine B/C-DNA sandwich-structured nanocomposites for intracellular miRNA biosensing. The nanoprobe was composed of NaYF4:Yb,Er@NaYF(4)upconversion nanoparticles (energy donor) with an amino functionalized mesoporous silica shell (NH2-mSiO(2)), rhodamine B (acceptor) loaded into the mesopores of NH2-mSiO(2), and the complementary sequences of target miRNA (denoted as C-DNA) acting as recognition species wrapped on the nanocomposite. Due to the LRET behavior between donors and acceptors, the loaded rhodamine B can quench the green upconversion emission of NaYF4:Yb,Er@NaYF(4)at 540 nm completely. Moreover, it can be released from the nanocomposite in the presence of target miRNA, which blocked the LRET behavior to "turn on" the green upconversion luminescence. Besides, as the unaffected red upconversion luminescence (at 660 nm) can be used as an internal standard to provide built-in correction for environmental effects, the intensity ratio of upconversion luminescence at 540 and 660 nm (I-540/I-660) was employed as the output signal to afford an accurate detection of target miRNA. Due to the biocompatibility, high photostability and low auto-fluorescence background, the nanoprobe was successfully utilized to diagnose the intracellular miRNA-21 expression in MCF-7 cellsviaupconversion fluorescence imaging. We envision that the proposed LBRU nanoprobe has great potential applications in early cancer diagnosis.