Towards a better recording of microtubule cytoskeletal spatial organization and dynamics in plant cells
Towards a better recording of microtubule cytoskeletal spatial organization and dynamics in plant cells
复制标题
更好地记录植物细胞中微管细胞骨架的空间组织和动力学。
DOI：
10.1111/jipb.12721
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发表时间：
2019-04-01
影响因子：
11.4
通讯作者：
Kong, Zhaosheng
中科院分区：
文献类型：
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作者：
Liu, Weiwei;Wang, Chaofeng;Kong, Zhaosheng
许多荧光标记物系目前可用于可视化活植物细胞中的微管（MT）结构和动力学，例如表达p35 S：：GFP-MBD或p35 S：：GFP-TUB 6的标记物。然而，这些MT标记系显示出明显的缺陷，影响植物生长或产生不稳定的荧光信号。在这里，开发了一系列新的标记系，包括pTUB 6：：VisGreen-TUB 6-表达系，其中TUB 6在其内源性调控元件的控制下，eGFP被更亮的荧光蛋白VisGreen取代。将两个不同的标记组合到一个表达载体中，构建了两个双标记株系。这些标记系在各种组织中产生明亮、稳定的荧光信号，并大大缩短了产生双标记系的筛选过程。这些新的标记系为MT研究提供了新的资源。
Numerous fluorescent marker lines are currently available to visualize microtubule (MT) architecture and dynamics in living plant cells, such as markers expressing p35S::GFP-MBD or p35S::GFP-TUB6. However, these MT marker lines display obvious defects that affect plant growth or produce unstable fluorescent signals. Here, a series of new marker lines were developed, including the pTUB6::VisGreen-TUB6-expressing line in which TUB6 is under the control of its endogenous regulatory elements and eGFP is replaced with VisGreen, a brighter fluorescent protein. Moreover, two different markers were combined into one expression vector and developed two dual-marker lines. These marker lines produce bright, stable fluorescent signals in various tissues, and greatly shorten the screening process for generating dual-marker lines. These new marker lines provide a novel resource for MT research.