ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in arabidopsis.
ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in arabidopsis.
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ABI4 通过调节拟南芥中脱落酸和赤霉素的生物合成来调节初级种子休眠
DOI：
10.1371/journal.pgen.1003577
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发表时间：
2013-06
期刊：
影响因子：
4.5
通讯作者：
Xie Q
中科院分区：
文献类型：
--
作者：
Shu K;Zhang H;Wang S;Chen M;Wu Y;Tang S;Liu C;Feng Y;Cao X;Xie Q
种子休眠性是农业生产中的一个重要经济性状。脱落酸（ABA）和赤霉素（GA）是调控休眠向萌发转变的主要因子，二者对这一过程具有拮抗作用。详细的监管机制，涉及ABA和GA之间的串扰，种子休眠的基础，需要进一步阐明。在这里，我们报告，ABI4正调节初级种子休眠，而负调节子叶绿化，通过介导的ABA和GA的生物合成。拟南芥abi4突变体的种子进行短期储存（一周或两周）发芽显着更快比野生型（WT），abi4子叶变绿显着更快比WT，而发芽和变绿的速度是相当的，当种子进行长期储存（六个月）。abi4干种子的ABA含量显著低于野生型，但层积后含量相当。因此，与WT相比，abi4种子的GA水平增加。进一步分析表明，abi4对GA生物合成抑制剂多效唑（PAC）的处理具有抗性，而OE-ABI 4对PAC敏感，外源GA可挽救OE-ABI 4的延迟萌发表型。通过qRT-PCR分析表明，在干种子和发芽种子中参与ABA和GA代谢的基因的表达对应于激素测量。此外，染色质免疫沉淀qPCR（ChIP-qPCR）和瞬时表达分析表明，ABI4抑制CYP707A1和CYP707A2的表达直接结合到这些启动子，和ABI4结合元件是必不可少的这种抑制。因此，进一步的遗传分析表明，abi4恢复了cyp707a1和cyp707a2的延迟发芽表型，并进一步挽救了ga1-t的非发芽表型。总而言之，这项研究表明，ABI 4是通过介导ABA和GA生物发生之间的平衡来调节种子初级休眠的关键因素。
Seed dormancy is an important economic trait for agricultural production. Abscisic acid (ABA) and Gibberellins (GA) are the primary factors that regulate the transition from dormancy to germination, and they regulate this process antagonistically. The detailed regulatory mechanism involving crosstalk between ABA and GA, which underlies seed dormancy, requires further elucidation. Here, we report that ABI4 positively regulates primary seed dormancy, while negatively regulating cotyledon greening, by mediating the biogenesis of ABA and GA. Seeds of the Arabidopsis abi4 mutant that were subjected to short-term storage (one or two weeks) germinated significantly more quickly than Wild-Type (WT), and abi4 cotyledons greened markedly more quickly than WT, while the rates of germination and greening were comparable when the seeds were subjected to longer-term storage (six months). The ABA content of dry abi4 seeds was remarkably lower than that of WT, but the amounts were comparable after stratification. Consistently, the GA level of abi4 seeds was increased compared to WT. Further analysis showed that abi4 was resistant to treatment with paclobutrazol (PAC), a GA biosynthesis inhibitor, during germination, while OE-ABI4 was sensitive to PAC, and exogenous GA rescued the delayed germination phenotype of OE-ABI4. Analysis by qRT-PCR showed that the expression of genes involved in ABA and GA metabolism in dry and germinating seeds corresponded to hormonal measurements. Moreover, chromatin immunoprecipitation qPCR (ChIP-qPCR) and transient expression analysis showed that ABI4 repressed CYP707A1 and CYP707A2 expression by directly binding to those promoters, and the ABI4 binding elements are essential for this repression. Accordingly, further genetic analysis showed that abi4 recovered the delayed germination phenotype of cyp707a1 and cyp707a2 and further, rescued the non-germinating phenotype of ga1-t. Taken together, this study suggests that ABI4 is a key factor that regulates primary seed dormancy by mediating the balance between ABA and GA biogenesis.
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影响因子：
6.9
作者：
Gerjets T;Scholefield D;Foulkes MJ;Lenton JR;Holdsworth MJ
通讯作者：
Holdsworth MJ
影响因子：
2.1
作者：
Hilhorst, HWM
通讯作者：
Hilhorst, HWM
影响因子：
7.2
作者：
FINKELSTEIN, RR
通讯作者：
FINKELSTEIN, RR
DOI：
10.1199/tab.0119
发表时间：
2008-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Bentsink, Leonie;Koornneef, Maarten
通讯作者：
Koornneef, Maarten
影响因子：
7.4
作者：
Kakizaki, Tomohiro;Matsumura, Hideo;Inaba, Takehito
通讯作者：
Inaba, Takehito