Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics
Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics
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DOI：
10.1167/iovs.10-6054
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发表时间：
2011-04-01
影响因子：
4.4
通讯作者：
Sjostrand, Johan
中科院分区：
文献类型：
--
作者：
Popovic, Zoran;Knutsson, Per;Sjostrand, Johan
目的.目的：应用高分辨率、宽视场、双共轭自适应光学（DCAO）成像仪对人视网膜中央凹毛细血管网络进行无创成像。用一种新的高分辨率、宽视野DCAO仪器对5名既往无眼部或神经系统疾病或手术史的健康受试者的中央凹毛细血管网络进行成像。使用手动程序定义每张图像中的中心凹无血管区（FAZ）。使用基于公开可用和定制编写的软件的自动算法来识别血管并提取形态FAZ和血管参数。在FAZ外的两个环形感兴趣区域（ROI）（距中央凹中心500 μ m和750 μ m外半径）和两个ROI内的上级、下级、颞部和鼻象限中计算毛细血管密度。平均FAZ面积为0.302 +/- 0.100 mm（2），内部ROI的平均毛细血管密度（长度/面积）为38.0 +/- 4.0 mm（-1），外部ROI为36.4 +/- 4.0 mm（-1）。ROI毛细血管密度差异无统计学意义。两个ROI内或不同ROI象限之间的象限毛细血管密度无显著差异。作者已经证明了一种非侵入性成像和半自动检测和分析中心凹毛细血管的技术。与其他研究相比，他们的方法产生的毛细血管密度低于组织学，但与目前的临床金标准荧光素血管造影结果相似。DCAO仪器增加的视野为中心凹毛细血管的高分辨率非侵入性临床成像开辟了新的可能性。（Invest Ophthalmol维斯科学。2011;52：2649-2655）DOI：10.1167/iovs.10-6054
PURPOSE. To demonstrate noninvasive imaging of human foveal capillary networks with a high-resolution, wide-field, dual-conjugate adaptive optics (DCAO) imaging instrument.METHODS. The foveal capillary networks of five healthy subjects with no previous history of ocular or neurologic disease or surgery were imaged with a novel high-resolution, wide-field DCAO instrument. The foveal avascular zone (FAZ) in each image was defined using a manual procedure. An automated algorithm based on publicly available and custom-written software was used to identify vessels and extract morphologic FAZ and vessel parameters. Capillary densities were calculated in two annular regions of interest (ROIs) outside the FAZ (500 mu m and 750 mu m outer radius from the foveal center) and in the superior, inferior, temporal, and nasal quadrants within the two ROIs.RESULTS. Mean FAZ area was 0.302 +/- 0.100 mm(2), and mean capillary density (length/area) in the inner ROI was 38.0 +/- 4.0 mm(-1) and 36.4 +/- 4.0 mm(-1) in the outer ROI. The difference in ROI capillary density was not significant. There was no significant difference in quadrant capillary density within the two ROIs or between quadrants irrespective of ROI.CONCLUSIONS. The authors have demonstrated a technique for noninvasive imaging and semiautomated detection and analysis of foveal capillaries. In comparison with other studies, their method yielded lower capillary densities than histology but similar results to the current clinical gold standard, fluorescein angiography. The increased field of view of the DCAO instrument opens up new possibilities for high-resolution noninvasive clinical imaging of foveal capillaries. (Invest Ophthalmol Vis Sci. 2011;52:2649-2655) DOI:10.1167/iovs.10-6054