Differential interactions of Id proteins with basic-helix-loop-helix transcription factors
Differential interactions of Id proteins with basic-helix-loop-helix transcription factors
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DOI：
10.1074/jbc.272.32.19785
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发表时间：
1997-08-08
影响因子：
4.8
通讯作者：
Prochownik, EV
中科院分区：
文献类型：
--
作者：
Langlands, K;Yin, XY;Prochownik, EV
三种Id蛋白的二聚化（Idl、Id 2和Id 3）与四种A类E蛋白质（E12、E47、E2-2和HE B）和两组B类蛋白，即肌生成调节因子（MRF：MyoD、肌细胞生成素、Myf-5和MRF 4/Myf-6）和造血因子（Scl/Tal-1、Tal-2和Lyl-1）。所有三种Ids都以高亲和力结合E蛋白，但在Ids和B类因子之间观察到更广泛的相互作用，Idl和Id 2与MyoD和Myf-5强相互作用，与肌细胞生成素和MRF 4/Myf-6弱相互作用，而Id 3与所有四种MRF弱相互作用。在免疫共沉淀和哺乳动物α-杂交分析中观察到类似的特异性。在Id和任何造血因子之间没有发现相互作用。每个Id能够破坏E蛋白-MyoD复合物在体内从肌肉肌酸激酶报告基因构建体反式激活的能力。最后，诱变实验显示Id 1和Id 3结合之间的差异映射到第一螺旋中的三个氨基酸和上游残基的小簇，因此，Id蛋白显示出与其所有潜在的二聚化伴侣相互作用的签名范围，并且可能在肌生成中发挥作用，这与造血不同。
Dimerization of three Id proteins (Idl, Id2, and Id3) with the four class A E proteins (E12, E47, E2-2, and HEB) and two groups of class B proteins, the myogenic regulatory factors (MRFs: MyoD, myogenin, Myf-5 and MRF4/Myf-6), and the hematopoietic factors (Scl/Tal-1, Tal-2, and Lyl-1) were tested in a quantitative yeast 2-hybrid assay, All three Ids bound with high affinity to E proteins, but a much broader range of interactions was observed between Ids and the class B factors, Idl and Id2 interacted strongly with MyoD;and Myf-5 and weakly with myogenin and MRF4/Myf-6, whereas Id3 interacted weakly with all four MRFs. Similar specificities were observed in co-immunoprecipitation and mammalian a-hybrid analyses, No interactions were found between the Ids and any of the hematopoietic factors, Each Id was able to disrupt the ability of E protein-MyoD complexes to transactivate from a muscle creatine kinase reporter construct in vivo, Finally, mutagenesis experiments showed that the differences between Idl and Id3 binding map to three amino acids in the first helix and to a small cluster of upstream residues, The Id proteins thus display a signature range of interactions with all of their potential dimerization partners and may play a role in myogenesis which is distinct from that in hematopoiesis.