The RIM101 pathway has a role in Saccharomyces cerevisiae adaptive response and resistance to propionic acid and other weak acids
The RIM101 pathway has a role in Saccharomyces cerevisiae adaptive response and resistance to propionic acid and other weak acids
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DOI：
10.1111/j.1567-1364.2008.00473.x
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发表时间：
2009-03-01
影响因子：
3.2
通讯作者：
Sa-Correia, Isabel
中科院分区：
文献类型：
--
作者：
Mira, Nuno P.;Lourenco, Artur B.;Sa-Correia, Isabel
在这项研究中，酿酒酵母RIM 101信号通路的生理功能扩展到碱性pH诱导的反应之外。转录因子Rim 101 p被证明是需要最大限度地耐受弱酸诱导的压力，在pH 4.0，但不发挥保护作用，对低pH值本身（范围4.5-2.5），当强酸被用作酸化剂。暴露于丙酸胁迫（pH 4.0）后酵母转录组的Rim 101 p依赖性改变包括先前描述的Rim 101 p调节子的基因，但也包括新的靶基因，特别是参与细胞壁β-1，6-葡聚糖合成的KNH 1和未表征的ORF YIL 029 c，两者都是最大丙酸抗性所需的。提供丙酸抗性的基因的聚类揭示了通过多泡体途径参与蛋白质催化剂的富集以及内部pH和液泡功能的稳态。在所有确定的丙酸抗性决定簇之间建立的相互作用网络的分析显示了RIM 101基因周围的相互作用的富集，并突出了参与RIM 101 p蛋白水解加工的蛋白质的作用。研究表明，RIM 101表达是抵消丙酸诱导的细胞质酸化以及适当的液泡酸化和细胞壁结构所必需的，这些对于这种食品防腐剂促进的稳健的适应性反应和应激抵抗力具有积极意义。
The physiological function of the Saccharomyces cerevisiae RIM101 signaling pathway is extended in this study beyond alkaline pH-induced responses. The transcription factor Rim101p is demonstrated to be required for maximal tolerance to weak acid-induced stress, at pH 4.0, but does not exert protection against low pH itself (range 4.5-2.5), when a strong acid is used as the acidulant. The Rim101p-dependent alterations of the yeast transcriptome following exposure to propionic acid stress (at pH 4.0) include genes of the previously described Rim101p regulon but also new target genes, in particular KNH1, involved in cellwall beta-1,6-glucan synthesis and the uncharacterized ORF YIL029c, both required for maximal propionic acid resistance. Clustering of the genes that provide resistance to propionic acid reveals the enrichment of those involved in protein catabolism through the multivesicular body pathway and in the homeostasis of internal pH and vacuolar function. The analysis of the network of interactions established among all the identified propionic acid resistance determinants shows an enrichment of interactions around the RIM101 gene and highlights the role of proteins involved in Rim101p proteolytic processing. RIM101 expression is shown to be required to counteract propionic acid-induced cytosolic acidification and for proper vacuolar acidification and cell wall structure, these having positive implications for a robust adaptive response and resistance to stress promoted by this food preservative.