Collimonas fungivorans gen. nov., sp nov., a chitinolytic soil bacterium with the ability to grow on living fungal hyphae
Collimonas fungivorans gen. nov., sp nov., a chitinolytic soil bacterium with the ability to grow on living fungal hyphae
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DOI：
10.1099/ijs.0.02920-0
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发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
van Veen, JA
中科院分区：
文献类型：
--
作者：
de Boer, W;Leveau, JHJ;van Veen, JA
使用多相方法描述了 22 种几丁质分解细菌分离株的系统发育位置，这些细菌分离株能够以几种土壤真菌完整的活菌丝为代价生长。这些分离物是在荷兰的微酸性沙丘土壤中发现的，是严格需氧的革兰氏阴性杆菌。在液体培养物中生长的细胞有鞭毛并具有菌毛。多种糖、醇、有机酸和氨基酸可以被代谢，而几种二糖和三糖不能用作底物。主要的细胞脂肪酸是C-16:0、C(16:1)omega7c和C(18:1)omega7c。 DNA G+C含量为57-62mol%。对近全长 16S rDNA 序列的分析表明，分离株彼此密切相关（> 98.6% 序列相似性），可归属于 β-变形菌门、“草酸杆菌科”、“伯克霍尔德菌目”。最密切相关的物种属于 Herbaspirillum 和 Janthinobacter 属，与分离株的 16S rDNA 序列相似性为 95.9-96.7%（Herbaspirillum 种）和 94.3-95.6%（Janthinobacter 种）。一些生理和生化特性表明分离株可以与这两个属清楚地区分开来。因此，建议本研究中描述的分离株是新属科利莫单胞菌属的代表。十一月基因组指纹 (BOX-PCR)、16S rDNA 模式的详细分析和分离株的生理特征 (Biolog) 揭示了四个亚群的存在。名称 Collimonas fungurans gen.十一月，SP。十一月已被分配给一个亚簇（四个分离株），该亚簇似乎位于新属的中心；其他亚群中的分离株暂定名为 Collimonas sp.。 Collimonas fungurans gen.的模式菌株。十一月，SP。十一月是 Ter6(T)（= NCCB 100033(T) = LMG 21973(T)）。
A polyphasic approach was used to describe the phylogenetic position of 22 chitinolytic bacterial isolates that were able to grow at the expense of intact, living hyphae of several soil fungi. These isolates, which were found in slightly acidic dune soils in the Netherlands, were strictly aerobic, Gram-negative rods. Cells grown in liquid cultures were flagellated and possessed pili. A wide range of sugars, alcohols, organic acids and amino acids could be metabolized, whereas several di- and trisaccharides could not be used as substrates. The major cellular fatty acids were C-16:0, C(16:1)omega7c and C(18:1)omega7c. DNA G + C contents were 57-62 mol%. Analysis of nearly full-length 16S rDNA sequences showed that the isolates were related closely to each other (> 98.6% sequence similarity) and could be assigned to the beta-Proteobacteria, family 'Oxalobacteraceae', order 'Burkholderiales'. The most closely related species belonged to the genera Herbaspirillum and Janthinobacterium, exhibiting 95.9-96.7% (Herbaspirillum species) and 94.3-95.6% (Janthinobacterium species) 16S rDNA sequence similarity to the isolates. Several physiological and biochemical properties indicated that the isolates could be distinguished clearly from both of these genera. Therefore, it is proposed that the isolates described in this study are representatives of a novel genus, Collimonas gen. nov. Genomic fingerprinting (BOX-PCR), detailed analysis of 16S rDNA patterns and physiological characterization (Biolog) of the isolates revealed the existence of four subclusters. The name Collimonas fungivorans gen. nov., sp. nov. has been given to one subcluster (four isolates) that appears to be in the centre of the novel genus; isolates in the other subclusters have been tentatively named Collimonas sp. The type strain of Collimonas fungivorans gen. nov., sp. nov. is Ter6(T) (= NCCB 100033(T) = LMG 21973(T)).