Autophagosome closure requires membrane scission.
Autophagosome closure requires membrane scission.
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DOI：
10.1080/15548627.2015.1091552
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发表时间：
2015-11-02
期刊：
影响因子：
13.3
通讯作者：
Dimova R
中科院分区：
文献类型：
--
作者：
Knorr RL;Lipowsky R;Dimova R
在巨自噬的细胞内过程中，一种膜结合的细胞器，即自噬体，重新产生。在细胞器的生命周期中，自噬膜的重塑是一个复杂的多步骤过程，涉及自噬膜拓扑结构的几个变化。在这里，我们专注于自噬体形成的最后一步，吞噬细胞的关闭，在此期间，内部和外部自噬膜成为独立的实体。我们认为，这种拓扑膜转换是一个膜断裂事件。令人惊讶的是，没有一个最近的评论将这个子步骤描述为膜断裂（或膜裂变）。相反，许多出版物暗示涉及膜融合。我们讨论了误解的潜在来源，并建议一致使用明确的术语“膜断裂”。
During the intracellular process of macroautophagy (hereafter autophagy), a membrane-bound organelle, the autophagosome, is generated de novo. The remodeling of the autophagic membrane during the life cycle of the organelle is a complex multistep process and involves several changes in the topology of the autophagic membrane. Here, we focus on the final step of autophagosome formation, the closure of the phagophore, during which the inner and outer autophagic membranes become separate entities. We argue that this topological membrane transformation is a membrane scission event. Surprisingly, not a single recent review describes this substep as membrane scission (or membrane fission). In contrast, a number of publications imply that membrane fusion is involved. We discuss the potential sources for misinterpretation and recommend to consistent use of the unambiguous term “membrane scission.”