Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats
Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats
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DOI：
10.1128/aem.69.9.5051-5059.2003
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发表时间：
2003-09-01
影响因子：
4.4
通讯作者：
Wagner-Döbler, I
中科院分区：
文献类型：
--
作者：
Allgaier, M;Uphoff, H;Wagner-Döbler, I
海洋玫瑰杆菌分支包含与未培养的 SAR83 簇相关的多个海洋细菌属，SAR83 簇是第二丰富的海洋超微型浮游生物谱系。该进化枝的培养代表在生理上是异质的，只有一些具有有氧无氧光合作用的能力，这一过程在世界海洋中具有潜在的巨大生态重要性。为了将系统发育与生态学联系起来，我们使用 16S rRNA 基因作为系统发育标记基因，研究了来自不同海洋生境（水样、生物膜、海带、硅藻和甲藻培养物）的玫瑰杆菌分支菌株的多样性。通过对细菌光合作用反应中心的pufLM基因进行PCR扩增和测序，在基因水平上确定有氧无氧光合作用的潜力，并通过检测细菌叶绿素（Bchl）a在生理水平上确定。大约的集合。通过 16S rRNA 基因定向多重 PCR 和测序，对 1,000 个海洋分离株进行了海洋玫瑰杆菌分支成员的筛选。发现的 42 个玫瑰杆菌分支分离株倾向于形成特定栖息地的亚群。 pufLM 基因在甲藻培养物的两组菌株中检测到，但在其他玫瑰杆菌分支分离株中均未检测到。第一组（DFL-12簇）内的菌株也合成了Bchl a。第二组（DFL-35簇）内的菌株形成了Roseovarius的新种，并且在此研究的条件下不产生Bchl a，从而证明了遗传方法对于筛选培养依赖性代谢性状的重要性。甲藻分离株的 pufLM 基因在系统发育上与 Betaproteobacteria 的 pufL 基因密切相关，证实了类似的先前观察结果，这些观察结果被解释为基因转移事件的迹象。
The marine Roseobacter clade comprises several genera of marine bacteria related to the uncultured SAR83 cluster, the second most abundant marine picoplankton lineage. Cultivated representatives of this clade are physiologically heterogeneous, and only some have the capability for aerobic anoxygenic photosynthesis, a process of potentially great ecological importance in the world's oceans. In an attempt to correlate phylogeny with ecology, we investigated the diversity of Roseobacter clade strains from various marine habitats (water samples, biofilms, laminariae, diatoms, and dinoflagellate cultures) by using the 16S rRNA gene as a phylogenetic marker gene. The potential for aerobic anoxygenic photosynthesis was determined on the genetic level by PCR amplification and sequencing of the pufLM genes of the bacterial photosynthesis reaction center and on the physiological level by detection of bacteriochlorophyll (Bchl) a. A collection of ca. 1,000 marine isolates was screened for members of the marine Roseobacter clade by 16S rRNA gene-directed multiplex PCR and sequencing. The 42 Roseobacter clade isolates found tended to form habitat-specific subclusters. The pufLM genes were detected in two groups of strains from dinoflagellate cultures but in none of the other Roseobacter clade isolates. Strains within the first group (the DFL-12 cluster) also synthesized Bchl a. Strains within the second group (the DFL-35 cluster) formed a new species of Roseovarius and did not produce Bchl a under the conditions investigated here, thus demonstrating the importance of genetic methods for screening of cultivation-dependent metabolic traits. The pufLM genes of the dinoflagellate isolates were phylogenetically closely related to pufL genes from Betaproteobacteria, confirming similar previous observations which have been interpreted as indications of gene transfer events.