EPIDERMAL-CELL FATE DETERMINATION IN ARABIDOPSIS - PATTERNS DEFINED BY A STEROID-INDUCIBLE REGULATOR
EPIDERMAL-CELL FATE DETERMINATION IN ARABIDOPSIS - PATTERNS DEFINED BY A STEROID-INDUCIBLE REGULATOR
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DOI：
10.1126/science.7939683
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发表时间：
1994-10-21
期刊：
影响因子：
56.9
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
LLOYD, AM;SCHENA, M;DAVIS, RW
拟南芥突变体 ttg 缺乏毛状体（表皮毛）和花青素色素。毛状体和花青素通过玉米转录调节因子 (R) 的组成型表达而恢复。 R-糖皮质激素受体嵌合蛋白的表达导致类固醇激素依赖性、条件性 R 等位基因在整个拟南芥植物中发挥作用。嵌合蛋白对激素脉冲的反应被用来定义发育中的叶表皮上毛状体形成的模式和时间。每个近轴表皮叶细胞分化成毛状体的概率均相同；有一个决定毛状体细胞命运的颞区，它以波的形式从发育中的叶子的尖端到基部进行。
The Arabidopsis mutant ttg lacks both trichomes (epidermal hairs) and anthocyanin pigments. Trichomes and anthocyanins are restored by the constitutive expression of the maize transcriptional regulator (R). The expression of an R-glucocorticoid receptor chimeric protein results in a steroid hormone-dependent, conditional allele of R that functions in whole Arabidopsis plants. The response of the chimeric protein to pulses of hormone was used to define the pattern ana timing of trichome formation on the developing leaf epidermis. Each adaxial epidermal leaf cell appears to have an equal probability of differentiating into a trichome; there is a temporal zone of decision for trichome cell fate that proceeds as a wave from the tip to the base of developing leaves.