Design of a Microfluidic Chip for Magnetic-Activated Sorting of One-Bead-One-Compound Libraries.
Design of a Microfluidic Chip for Magnetic-Activated Sorting of One-Bead-One-Compound Libraries.
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用于一珠一化合物文库磁激活分选的微流控芯片的设计。
DOI：
10.1021/acscombsci.5b00180
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发表时间：
2016
影响因子：
--
通讯作者：
Lawler,SeanE
中科院分区：
文献类型：
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作者：
Cho,Choi-Fong;Lee,Kyungheon;Speranza,Maria-Carmela;Bononi,FernandaC;Viapiano,MarianoS;Luyt,LeonardG;Weissleder,Ralph;Chiocca,EAntonio;Lee,Hakho;Lawler,SeanE
Molecular targeting using ligands specific to disease markers has shown great promise for early detection and directed therapy. Bead-based combinatorial libraries have served as powerful tools for the discovery of novel targeting agents. Screening platforms employing magnetic capture have been used to achieve rapid and efficient identification of high-affinity ligands from one-bead-one-compound (OBOC) libraries. Traditional manual methodologies to isolate magnetized “hit” beads are tedious and lack accuracy, and existing instruments to expedite bead sorting tend to be costly and complex. Here, we describe the design and construction of a simple and inexpensive microfluidic magnetic sorting device using standard photolithography and soft lithography approaches to facilitate high-throughput isolation of magnetized positive hit beads from combinatorial libraries. We have demonstrated that the device is able to sort magnetized beads with superior accuracy compared to conventional manual sorting approaches. This chip offers a very convenient yet inexpensive alternative for screening OBOC libraries.
