Reversible Conduction Block in Isolated Toad Sciatic Nerve by Emulsified Isoflurane
Reversible Conduction Block in Isolated Toad Sciatic Nerve by Emulsified Isoflurane
复制标题
乳化异氟烷对离体蟾蜍坐骨神经的可逆传导阻滞
DOI：
10.1213/ane.0b013e3181d2732f
复制
发表时间：
2010-04-01
影响因子：
5.7
通讯作者：
Li, Guo-hua
中科院分区：
文献类型：
--
作者：
Li, Zhuo;Yang, Jing;Li, Guo-hua
背景：研究表明，局部使用挥发性麻醉药可产生局部麻醉作用。本研究通过测量离体蟾蜍坐骨神经的复合神经动作电位（CNAP）参数，评价乳化异氟烷（EI）的神经传导阻滞特性，并与1%利多卡因的神经阻滞作用进行比较。方法：选取100只离体蟾蜍坐骨神经，随机分为10组，每组10只，给予2%～8%EI（v/v）（EI8组等）， 1%利多卡因、30%英脱利匹特(R)(中国江苏无锡华瑞药房)和林格溶液(RS)分别作用10分钟。然后清洗所有神经并用RS浸泡10分钟和30分钟。神经传导阻滞效果以每分钟细胞外记录技术记录的CNAP参数来表示。 结果：结果显示，El和利多卡因使CNAP的负波幅降低（P < 0.05），并且在某些时间点（D7-W3）CNAP的传导速度也降低（P < 0.05）。 RS清洗后，2个参数逐渐恢复。 El 引起的 2 个参数的变化比利多卡因产生的起效速度更慢，恢复更快（7 分钟与 1 分钟以及 9 分钟与 30 分钟）。 El引起的神经阻滞呈剂量依赖性（P < 0.05），El的半数抑制浓度为5.46%。结论：El产生完全可逆的、剂量依赖的神经传导抑制作用，与利多卡因相比，起效较慢，恢复较快。 （麻醉安娜 Ig 2010；110：1024-9）
BACKGROUND: Studies have shown that the local use of volatile anesthetics can produce local anesthetic effects. We designed this study to evaluate the characteristics of nerve conduction block of emulsified isoflurane (EI) and compare its nerve blockade with 1% lidocaine, by measuring compound nerve action potential (CNAP) parameters in isolated toad sciatic nerve.METHODS: One hundred isolated toad sciatic nerves were selected and randomly assigned to 10 groups of 10 each, administered 2% to 8% EI (v/v) (EI8 group, etc.), 1% lidocaine, 30% Intralipid (R) (Huarui Pharmacy, Wuxi, Jiangsu, China), and Ringer solution (RS) for 10 minutes, respectively. All nerves were then washed and soaked with RS for 10 minutes and 30 minutes. The nerve conduction block effect was represented by CNAP parameters that were recorded by an extracellular recording technique per minute.RESULTS: The results showed that the negative amplitudes of CNAP were decreased by El and lidocaine (P < 0.05), and the conduction velocities of CNAP were also decreased at some time points (D7-W3) (P < 0.05). After RS washing, the 2 parameters recovered gradually. The changes in the 2 parameters induced by El had slower onset rates and faster recoveries than those produced by lidocaine (7 minutes vs 1 minute and 9 minutes vs 30 minutes). The nerve blockade induced by El was dose dependent (P < 0.05), and the half maximal inhibition concentration of El was 5.46%.CONCLUSIONS: El produced completely reversible and dose-dependent nerve conduction inhibition, which had slower onset and faster recovery compared with those produced by lidocaine. (Anesth Ana Ig 2010;110:1024-9)