Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation
Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation
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DOI：
10.1021/ac981367d
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发表时间：
1999-05-15
影响因子：
7.4
通讯作者：
Rawson, K
中科院分区：
文献类型：
--
作者：
Ho, WO;Krause, S;Rawson, K
酶催化聚合物转化-电化学交流阻抗检测法已用于血清样品中尿素和肌酐的测定。一种聚合物，基于聚（甲基乙烯基醚）/马来酸酐改性的酯化与正辛醇，这是稳定的pH值为7.4，这是迅速转变，响应碱性pH值的变化，连接到酶之间的酶促反应和尿素或肌酸酐脱亚胺酶和肌酸酐，以产生一次性传感器系统。将聚合物丝网印刷到叉指型丝网印刷碳电极上，并且电极覆盖有含有相关酶的吸收垫。血清样品的应用，“加标”与尿素或肌酸酐，导致快速聚合物转化，并在聚合物涂层的电极的电容的结果变化是分析物浓度依赖性的。从动态石英晶体微天平测量获得的聚合物转化的机制的额外信息。
Enzyme-catalyzed polymer transformation with electrochemical ac impedance detection has been employed for the measurement of urea and creatinine in serum samples. A polymer, based on poly(methylvinyl ether)/maleic anhydride modified by esterification with n-octanol, which is stable at pH 7.4 and which is transformed rapidly in response to alkaline pH changes, was linked to enzymatic reactions between urease and urea or creatinine deiminase and creatinine to produce a disposable sensor system. The polymer was screen-printed onto interdigitated screen-printed carbon electrodes and the electrodes overlaid with absorbent pads containing the relevant enzyme. Application of serum samples, "spiked" with either urea or creatinine, resulted in rapid polymer transformation, and resultant changes in the capacitance of the polymer-coated electrodes were analyte-concentration dependent. Additional information on the mechanisms of polymer transformation was obtained from dynamic quartz crystal microbalance measurements.