Biosynthesis of staurosporine, 2. Incorporation of tryptophan.
Biosynthesis of staurosporine, 2. Incorporation of tryptophan.
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星形孢菌素的生物合成，2.色氨酸的掺入。
DOI：
10.1021/np50059a013
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发表时间：
1988
影响因子：
5.1
通讯作者：
Cordell,GA
中科院分区：
文献类型：
--
作者：
Meksuriyen,D;Cordell,GA
在确定了在STauros-poreus中产强细胞毒性抗生素STauroporine的时间进程、培养基和营养参数之后，STauroporine与单标记物和双标记放射性前体的生物合成研究证实，一个或两个单位的色氨酸被有效地结合。根据稳定同位素掺入实验后星形孢菌素的~(13)C-核磁共振谱，确定苷元部分是由两个单位的色氨酸衍生而成，碳骨架完整。
Following studies to define thetime course, media, and nutrient parameters for the production of the potent cytotoxic antibiotic staurosporine [1] in Streptomyces stauros-poreus, biosynthetic studies of staurosporine with singly-and doubly-labeled radioactive precur-sors established that either one or two units of tryptophan was incorporated efficiently. From the 13C-nmr spectrum of staurosporine subsequent to stable isotope incorporation experiments, it was established that the aglycone moiety was derived from two units of tryptophan with the car-bon skeleton incorporated intact.