Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism.
Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism.
复制标题
白术多糖与氧化锌纳米颗粒的结合：表征、免疫效应及机制。
DOI：
10.3389/fnut.2022.992502
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发表时间：
2022
影响因子：
5
通讯作者：
Pang, Huan
中科院分区：
文献类型：
--
作者：
Bo, Ruonan;Liu, Xiaopan;Wang, Jing;Wei, Simin;Wu, Xinyue;Tao, Ya;Xu, Shuya;Liu, Mingjiang;Li, Jingui;Pang, Huan
关键词：
Atractylodes macrocephala Koidz (A. macrocephala) has been used both as a traditional medicine and functional food for hundreds of years in Asia. And it has a variety of biological activities, such as enhancing the ability of immunity and modulating effect on gastrointestinal motility. In this study, a water-soluble polysaccharide with molecular weight of 2.743 × 103 Da was isolated from the root of A. macrocephala. Polysaccharide from A. macrocephala (AMP) consisted of arabinose, galactose, glucose, xylose, mannose, ribose, galactose uronic acid, glucose uronic acid, with a percentage ratio of 21.86, 12.28, 34.19, 0.43, 0.92, 0.85, 28.79, and 0.67%, respectively. Zinc plays an important role in immune system. Therefore, we supposed that AMP binding with zinc oxide (ZnO) nanoparticles (AMP-ZnONPs) might be an effective immunostimulator. AMP-ZnONPs was prepared by Borch reduction, and its structural features were characterized by Scanning Electron Microscope (SEM), Transmission electron microscope (TEM), TEM-energy dispersive spectroscopy mapping (TEM-EDS mapping), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS), X-ray diffraction (XRD), particle size and zeta-potential distribution analysis. Then, its immunostimulatory activity and the underlying mechanism were evaluated using RAW264.7 cells. The results showed that AMP-ZnONPs remarkably promoted cell proliferation, enhanced phagocytosis, the release of nitric oxide (NO), cytokines (IL-6 and IL-1β) and the expression of co-stimulatory molecules (CD80, CD86 and MHCII). Moreover, AMP-ZnONPs could promote the expression of Toll-like receptor 4 (TLR4), Myeloid differentiation factor 88 (MyD88), TNF receptor associated factor 6 (TRAF6), phospho-IκBα (P-IκBα) and phospho-p65 (P-p65), and TLR4 inhibitor (TAK242) inhibited the expression of these proteins induced by AMP-ZnONPs. Therefore, AMP-ZnONPs activated macrophages by TLR4/MyD88/NF-κB signaling pathway, indicating that AMP-ZnONPs could act as a potential immunostimulator in medicine and functional food.
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影响因子：
6.7
作者：
Hawash, Mohammed M. A.;Kahraman, Deniz Cansen;Baytas, Sultan Nacak
通讯作者：
Baytas, Sultan Nacak
影响因子：
9.5
作者：
Meng, Shangjun;Hong, Ying;Dong, Xiaochen
通讯作者：
Dong, Xiaochen
DOI：
10.1016/j.ijbiomac.2020.02.018
发表时间：
2020-04-15
影响因子：
8.2
作者：
Ji, Xiaolong;Hou, Chunyan;Liu, Yanqi
通讯作者：
Liu, Yanqi
黄秋葵多糖-2通过TLR2/4介导的信号转导途径对RAW264.7细胞的激活起着至关重要的作用
影响因子：
5.6
作者：
Chen, Yun;Zhou, Ruigang;Chen, Huricha
通讯作者：
Chen, Huricha
影响因子：
--
作者：
Du, Gao-Xiang;Xue, Qiang;Li, Zhaohui
通讯作者：
Li, Zhaohui