Generation of monodisperse alginate microbeads and in situ encapsulation of cell in microfluidic device
Generation of monodisperse alginate microbeads and in situ encapsulation of cell in microfluidic device
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DOI：
10.1007/s10544-007-9098-7
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发表时间：
2007-12-01
影响因子：
2.8
通讯作者：
Lee, Chang-Soo
中科院分区：
文献类型：
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作者：
Choi, Chang-Hyung;Jung, Jae-Hoon;Lee, Chang-Soo
描述了一种利用混沌混合原位制备单分散藻酸盐水凝胶的微流控方法。将包含藻酸盐和作为交联剂的钙的水性液滴形成为不混溶的连续相，然后液滴中的藻酸盐和钙接触并快速混合。通过微流控装置中液滴的混沌混合，原位实现水凝胶的凝胶化。重要的操作参数包括：毛细管数（Ca）和连续相的流速，这主要影响三个独特的流态，如波动，稳定液滴，层流的形成。在稳定形成液滴的情况下，在微流控装置中产生具有窄尺寸分布（CV低于3%）的单分散藻酸盐微珠，并且通过改变流速、粘度和界面张力可以容易地调节微珠的尺寸，范围为60至95 μ m。此外，该方法可应用于酵母细胞在具有高单分散性的海藻酸盐水凝胶中的包封。这种用于生产单分散水凝胶和生物分子封装的简单微流体技术显示出在生物传感器、细胞传感器、药物递送系统和细胞移植应用中的强大潜力。
A microfluidic method for the in situ production of monodispersed alginate hydrogels using chaotic mixing is described. Aqueous droplets comprising of alginate and calcium as a cross-linking agent were formed as an immiscible continuous phase, and then the alginate and calcium in the droplet came into contact and were rapidly mixed. Gelation of the hydrogel was achieved in situ by the chaotic mixing of the droplets in the microfluidic device. Important operating parameters included: the capillary number (Ca) and the flow rate of the continuous phase, which mainly influenced the formation of three distinctive flow regimes, such as fluctuation, stable droplets, and laminar flow. Under the stable formation of droplets regime, monodispersed alginate microbeads having a narrow size distribution (below 3% of CV) were produced in the microfluidic device and the size of the microbeads, ranging from 60 to 95 mu m, could be easily modulated by varying the flow rate, viscosity, and interfacial tension. In addition, this approach can be applied to the encapsulation of yeast cells in alginate hydrogels with a high monodispersity. This simple microfluidic technique for the production of monodispersed hydrogels and encapsulation of biomolecules shows strong potential for use in biosensors, cell sensors, drug delivery systems, and cell transplantation applications.