Apoptotic and proliferative activities in first trimester placentae
Apoptotic and proliferative activities in first trimester placentae
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DOI：
10.1053/plac.1998.0375
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发表时间：
1999-03-01
期刊：
影响因子：
3.8
通讯作者：
Cheung, ANY
中科院分区：
文献类型：
--
作者：
Chan, CCW;Lao, TT;Cheung, ANY
细胞凋亡和细胞增殖被广泛认为是共同维持组织稳态的重要生理过程。分别采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）和Ki-67抗原抗体对38例孕早期胎盘的凋亡和增殖过程进行定量。在14例中，通过鉴定凋亡的形态学特征，用苏木精和伊红染色的切片重复估计凋亡指数。我们发现，通过两种方法获得的指数彼此相关性很好，尽管使用苏木素和伊红染色切片获得的比率较低。细胞凋亡主要集中在合体滋养细胞中，并伴有纤维蛋白样沉积，而增殖活性仅限于细胞滋养细胞和基质细胞。增殖指数与胎龄及凋亡指数呈显著负相关。虽然细胞凋亡的程度随着孕龄的增加而降低，但相关性没有统计学意义。这些发现提供了一个潜在的解释绒毛重塑在滋养层细胞入侵在早孕。(C)小行星1999 W. B。Saunders Company Ltd
Apoptosis and cell proliferation are widely recognized to be important physiological processes which together maintain tissue homeostasis. The apoptotic and proliferative processes in 38 first trimester placentae were quantified using terminal deoxynucleotidyl transferase mediated dUTP nick end-labelling (TUNEL) and an antibody against Ki-67 antigen, respectively. In 14 cases, the estimation of apoptotic index was repeated with sections stained with haematoxylin and eosin by identifying the morphological features of apoptosis. We found that the indices obtained by either method correlated well with each other although a lower rate was obtained with the haematoxylin and eosin stained sections. Apoptosis was found in clusters mainly in syncytiotrophoblasts in association with fibrinoid deposits while proliferating activity was limited to cytotrophoblasts and stromal cells. A significant inverse correlation was observed between the proliferative index and gestational age as well as the apoptotic index. While the extent of apoptosis decreased with advanced gestational age, the correlation was not statistically significant. These findings provide a potential explanation for villous remodelling during trophoblastic invasion in early pregnancy. (C) 1999 W. B. Saunders Company Ltd.