Ultra-HPLC method for quality and adulterant assessment of steviol glycosides sweeteners - Stevia rebaudiana and stevia products.
Ultra-HPLC method for quality and adulterant assessment of steviol glycosides sweeteners - Stevia rebaudiana and stevia products.
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用于甜菊醇糖苷甜味剂（甜叶菊和甜菊产品）质量和掺假评估的超高效液相色谱法。
DOI：
10.1080/19440049.2015.1021863
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Khan,IkhlasA
中科院分区：
文献类型：
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作者：
Wang,Yan-Hong;Avula,Bharathi;Tang,Wenzhao;Wang,Mei;Elsohly,MahmoudA;Khan,IkhlasA
甜菊糖产品被宣传为零卡路里甜味剂。葡萄糖不应该是本产品的内在成分，但在初步研究中已从一些甜叶菊产品中鉴定出。建立了超高效液相色谱-紫外分光光度法测定甜菊糖产品中葡萄糖含量的方法。在甜菊糖产物与1-苯基-3-甲基-5-吡唑啉酮（PMP）反应后，分析PMP衍生物，并且在35种产物中的7种中发现葡萄糖在0.3-91.5%（w/w）的范围内。两种产物SPR-12和SPR-27显示出显著量的葡萄糖，分别为61.6%和91.5%。此外，建立了超高效液相色谱-紫外蒸发光散射检测器（ELSD）法测定甜菊及其相关产品中莱鲍迪苷A、甜菊苷、莱鲍迪苷D、杜克苷A和甜菊双糖苷的含量。在12分钟运行中，基线分离五种甜菊醇糖苷。ELSD和紫外（UV）检测结果相当。对LC方法的线性、重复性、准确度、检测限（LOD）和定量限（LOQ）进行了验证。对于甜菊醇糖苷，发现LOD和LOQ分别小于10和30 μg ml-1。日内和日间分析的RSD小于2.5%，回收率为90- 94%。对于葡萄糖的PMP衍生物，LOD和LOQ分别为0.01和0.05 μg ml-1。方法的重复性（RSD）小于2.6%，回收率为98.6- 101.7%。该方法可用于S. rebaudiana和甜菊醇糖苷甜味剂（甜叶菊产品）。
Stevia products are advertised as a zero-calorie sweetener. Glucose should not be an intrinsic component of this product, but it has been identified from some of stevia products in a preliminary study. An UHPLC-UV method was developed for the quantitative determination of glucose from stevia products. After stevia products reacted with 1-phenyl-3-methyl-5-pyrazolone (PMP), PMP derivatives were analysed and glucose was found in seven out of 35 products in the range 0.3–91.5% (w/w). Two products, SPR-12 and SPR-27, showed remarkable amounts of glucose at 61.6% and 91.5%, respectively. In addition, an UHPLC-UV-evaporative light-scattering detector (ELSD) method was developed for the quantitative determination of rebaudioside A, stevioside, rebaudioside D, dulcoside A and steviolbioside fromSteviarebaudianaand related products. In a 12 min run, five steviol glycosides were baseline-separated. ELSD and ultraviolet (UV) detections showed comparable results. The LC methods were validated for linearity, repeatability, accuracy, limits of detection (LOD) and limits of quantification (LOQ). For steviol glycosides, the LODs and LOQs were found to be less than 10 and 30 μg ml–1, respectively. The RSD for intra- and inter-day analyses was less than 2.5%, and the recovery was 90–94%. For PMP derivative of glucose, the LOD and LOQ were 0.01 and 0.05 μg ml–1, respectively. Repeatability (RSD) was less than 2.6%; recovery was 98.6–101.7%. The methods are useful for the identification, quality assurance, and adulterant assessment ofS. rebaudianaand steviol glycosides sweeteners (stevia products).