Multiphoton redox ratio imaging for metabolic monitoring in vivo.
Multiphoton redox ratio imaging for metabolic monitoring in vivo.
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DOI：
10.1007/978-1-60761-411-1_11
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ramanujam, Nirmala
中科院分区：
文献类型：
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作者：
Skala, Melissa;Ramanujam, Nirmala
活组织中细胞水平的代谢监测对于了解细胞功能、疾病过程和潜在治疗非常重要。NADH和FAD（分别为电子传递链中的主要电子供体和受体）的相对量的多光子成像提供了一种用于在体内三维中以高分辨率监测细胞代谢活性的非侵入性方法。NADH和FAD是内源性组织荧光团，因此该方法不需要外源性染色或组织切除。我们描述了在体多光子氧化还原比成像的原理和协议。
Metabolic monitoring at the cellular level in live tissues is important for understanding cell function, disease processes and potential therapies. Multiphoton imaging of the relative amounts of NADH and FAD (the primary electron donor and acceptor, respectively, in the electron transport chain) provides a non-invasive method for monitoring cellular metabolic activity with high resolution in three dimensions in vivo. NADH and FAD are endogenous tissue fluorophores, and thus this method does not require exogenous stains or tissue excision. We describe the principles and protocols of multiphoton redox ratio imaging in vivo.