HEPARIN PROTECTS BASIC AND ACIDIC FGF FROM INACTIVATION
HEPARIN PROTECTS BASIC AND ACIDIC FGF FROM INACTIVATION
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DOI：
10.1002/jcp.1041280317
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发表时间：
1986-09-01
影响因子：
5.6
通讯作者：
CHENG, J
中科院分区：
文献类型：
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作者：
GOSPODAROWICZ, D;CHENG, J
已经分析了肝素或己糖醛基己糖胺聚糖硫酸盐 (HHS-4) 保护碱性或酸性成纤维细胞生长因子 (FGF) 免受酸或热失活的能力。新鲜制备的碱性和酸性 FGF 都会刺激暴露于补充有转铁蛋白和胰岛素的培养基中的幼仓鼠肾 (BHK-21) 细胞的生长。新鲜制备的碱性 FGF 的效力比酸性 FGF 强 10 倍。培养基中添加肝素降低了碱性 FGF 的效力，同时增强了酸性 FGF 的效力。 FGF在-80℃下储存后。 C，观察到碱性和酸性 FGF 的效力均下降。当将肝素添加到培养基中时，增强了它们的活性，其活性与新鲜制备的碱性 FGF 相似。为了测试肝素是否可以保护碱性或酸性FGF免于失活，将两种有丝分裂原暴露于酸性条件（1%三氟乙酸，pH 1.08，2小时）或热（65°C，5分钟），这使碱性或酸性FGF失活。当在肝素或 HHS-4 存在的情况下进行此类处理时，碱性和酸性 FGF 保留了其效力。肝素和 HHS-4 对碱性和酸性 FGF 生物活性的影响确实具有保护性，因为在丝裂原失活后添加它们没有任何影响。只有当细胞暴露于高浓度的肝素或 HHS-4 时，才能观察到受保护的有丝分裂原或失活的有丝分裂原的生物活性增强。这表明肝素和 HHS-4 除了保护 FGF 免于失活外，还在另一个尚未确定的位点发挥作用。
The ability of heparin or that of hexuronyl hexosaminoglycan sulfate (HHS-4) to protect basic or acidic fibroblast growth factor (FGF) from acid or heat inactivation has been analyzed. Both freshly prepared basic and acidic FGF stimulate the growth of baby hamster kidney (BHK-21) cells exposed to medium supplemented with transferrin and insulin. Freshly prepared basic FGF was 10 fold more potent than acidic FGF. The addition of heparin to the medium decreased the potency of basic FGF while it potentiated that of acidic FGF. Upon storage of FGF at -80.degree. C, a decline in potency of both basic and acidic FGF was observed. Heparin, when added to the medium, potentiated their activities, which became similar to that of freshly prepared basic FGF. In order to test whether heparin could protect basic or acidic FGF from inactivation, both mitogens were exposed to acid conditions (1% trifluoroacetic acid, pH 1.08, 2 h) or heat (65.degree.C, 5 min) which inactivate basic or acidic FGF. When exposed to such treatment in the presence of heparin or HHS-4, basic and acidic FGF retained their potency. The effect of heparin and HHS-4 on the bioactivity of basic and acidic FGF is truly of a protective nature, since they had no effect when added after inactivation of the mitogens. Potentiation of the bioactivity of the protected mitogens or of the inactivated one could only be observed when cells were exposed to high heparin or HHS-4 concentrations. This indicates that heparin and HHS-4, in addition to protecting FGF from inactivation, also acts at another locus, as yet unidentified.