Generation of Periodic Enzyme Patterns by Soft Lithography and Activity Imaging by Scanning Electrochemical Microscopy
Generation of Periodic Enzyme Patterns by Soft Lithography and Activity Imaging by Scanning Electrochemical Microscopy
复制标题
通过软光刻和扫描电化学显微镜活性成像生成周期性酶模式
作者：
T. Wilhelm;G. Wittstock
用不同的软光刻方法在多晶金和玻璃表面形成了葡萄糖氧化酶(GOX)和辣根过氧化物酶(HRP)的微图形。用非接触式扫描力显微镜对图形进行了分析。用扫描电化学显微镜对固定化图案的活性进行了局部分辨分析。已试验的方法如下：(I)在金上微接触印刷十八硫醇，然后化学吸附胱胺氯化物，并与戊二醛的混合物孵育，形成图案化的交联和接枝酶凝胶；(Ii)在金上微接触印刷十八硫醇，然后化学吸附热休克蛋白，其中通过S-乙酰硫醇酸-N-琥珀酰亚胺酯修饰引入了硫醇基团；(Iii)将Gox与N-(3-dimethylaminopropyl)-N‘-ethylcarbodiimide盐酸盐( )的混合物应用于邮票并与胺化玻璃表面接触；(Iv)递送...
Micropatterns of the enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP) have been formed on polycrystalline gold and glass surfaces using different soft-lithographic approaches. The patterns have been analyzed by noncontact scanning force microscopy. The activities of the immobilized patterns were probed with local resolution by scanning electrochemical microscopy. The following approaches have been tested: (i) microcontact printing of octadecanethiol on gold, followed by chemisorption of cystaminium chloride and incubation of a mixture of HRP and glutaraldehyde to form a patterned cross-linked and grafted enzyme gel; (ii) microcontact printing of octadecanethiol on gold followed by chemisorption of HRP into which an thiol group had been introduced by modification with S-acetylthioglycolic acid-N-succinimidyl ester; (iii) application of a mixture of GOx with N-(3-dimethylaminopropyl)-N‘-ethylcarbodiimide hydrochloride (EDAC) to a stamp and contact with an aminated glass surface; (iv) delivery...