The GTPase-activating protein Rga1 interacts with Rho3 GTPase and may regulate its function in polarized growth in budding yeast.
The GTPase-activating protein Rga1 interacts with Rho3 GTPase and may regulate its function in polarized growth in budding yeast.
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DOI：
10.1371/journal.pone.0123326
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Gao XD
中科院分区：
文献类型：
--
作者：
He F;Nie WC;Tong Z;Yuan SM;Gong T;Liao Y;Bi E;Gao XD
In budding yeast, Rga1 negatively regulates the Rho GTPase Cdc42 by acting as a GTPase-activating protein (GAP) for Cdc42. To gain insight into the function and regulation of Rga1, we overexpressed Rga1 and an N-terminally truncated Rga1-C538 (a.a. 538-1007) segment. Overexpression of Rga1-C538 but not full-length Rga1 severely impaired growth and cell morphology in wild-type cells. We show that Rga1 is phosphorylated during the cell cycle. The lack of phenotype for full-length Rga1 upon overexpression may result from a negative regulation by G1-specific Pho85, a cyclin-dependent kinase (CDK). From a high-copy suppressor screen, we isolated RHO3, SEC9, SEC1, SSO1, SSO2, and SRO7, genes involved in exocytosis, as suppressors of the growth defect caused by Rga1-C538 overexpression. Moreover, we detected that Rga1 interacts with Rho3 in two-hybrid and bimolecular fluorescence complementation (BiFC) assays. Rga1 preferentially interacts with the GTP-bound form of Rho3 and the interaction requires the GAP domain and additional sequence upstream of the GAP domain. Our data suggest that the interaction of Rga1 with Rho3 may regulate Rho3’s function in polarized bud growth.
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DOI：
10.1083/jcb.200109062
发表时间：
2002-01-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gladfelter AS;Bose I;Zyla TR;Bardes ES;Lew DJ
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影响因子：
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作者：
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Brennwald, PJ
影响因子：
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DOI：
10.1083/jcb.200212040
发表时间：
2003-06-23
期刊：
The Journal of cell biology
影响因子：
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通讯作者：
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DOI：
10.1083/jcb.146.1.125
发表时间：
1999-07-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
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Brennwald P