Effects of growth hormone on the salmon pituitary proteome
Effects of growth hormone on the salmon pituitary proteome
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DOI：
10.1016/j.jprot.2011.12.009
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发表时间：
2012-03-16
影响因子：
3.3
通讯作者：
Mori, Tsukasa
中科院分区：
文献类型：
--
作者：
Kurata, Yoichi;Kimura, Yayoi;Mori, Tsukasa
生长激素1（GH 1）是一种垂体激素，在生长调节中起着关键作用。过量的GH 1处理和GH 1转基因的过表达都促进鲑鱼的生长，但这些动物在生存力、生育力和代谢方面表现出生理异常，这可能与垂体功能有关。然而，在垂体中诱导的过量GH 1的分子动力学仍然未知。在这项研究中，我们进行iTRAQ蛋白质组分析的鲑鱼垂体，有和没有过量的GH 1治疗，并发现，与内分泌系统，代谢，细胞生长和增殖相关的蛋白质的表达水平在GH 1处理的垂体改变。具体而言，垂体激素催乳素（2.29倍）和生长抑素α（0.14倍）发生显著变化。这一结果得到证实的蛋白质组和转录组分析垂体GH 1转基因（GH 1-Tg）鲑鱼。GH 1-Tg amago鲑鱼垂体中的蛋白质和基因表达的动态与过量GH 1处理的垂体相似。我们的研究结果表明，不仅过量的GH 1激素，但也在其他垂体激素的数量变化，可能是必不可少的异常生长的amago鲑鱼。这些数据将有助于今后提高鱼类养殖的生产力。(C)2011 Elsevier B. V.保留所有权利。
Growth hormone 1 (GH1), a pituitary hormone, plays a key role in the regulation of growth. Both excess GH1 treatment and overexpression of a GH1 transgene promote growth of salmon, but these animals exhibit physiological abnormalities in viability, fertility and metabolism, which might be related to pituitary function. However, the molecular dynamics induced in the pituitary by excess GH1 remain unknown. In this study, we performed iTRAQ proteome analysis of the amago salmon pituitary, with and without excess GH1 treatment, and found that the expression levels of proteins related to endocrine systems, metabolism, cell growth and proliferation were altered in the GH1-treated pituitary. Specifically, pituitary hormone prolactin (2.29 fold), and somatolactin alpha (0.14 fold) changed significantly. This result was confirmed by proteome and transcriptome analyses of pituitary from the GH1-transgenic (GH1-Tg) amago salmon. The dynamics of protein and gene expression in the pituitary of GH1-Tg amago salmon were similar to those of pituitary treated with excess GH1. Our findings suggest that not only excess GH1 hormone, but also the quantitative changes in other pituitary hormones, might be essential for the abnormal growth of amago salmon. These data will be useful in future attempts to increase the productivity of fish farming. (C) 2011 Elsevier B.V. All rights reserved.