Detection and characterization of emerging relapsing fever pathogen, Borrelia miyamotoi, from Ixodes ricinus tick on rural Trakya (Thrace) region of north western Turkey
Detection and characterization of emerging relapsing fever pathogen, Borrelia miyamotoi, from Ixodes ricinus tick on rural Trakya (Thrace) region of north western Turkey
复制标题
土耳其西北部特拉基亚（色雷斯）农村地区蓖麻硬蜱中新出现的回归热病原体宫本疏螺旋体的检测和特征分析
DOI：
10.1089/vbz.2016.2012
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Masuzawa T
中科院分区：
文献类型：
--
作者：
Sakakibara Y;Sen E;Sato K;Kawabata H;Ohashi N;Masuzawa T
采用TaqMan-PCR、巢式PCR、鞭毛蛋白基因（flaB）、16 S和23 S rDNA基因间隔区（IGS）和测序技术，检测了来自土耳其西北部伊斯坦布尔和Kirklareli农村的蓖麻硬蜱中的回归热病原体宫本疏螺旋体（Borrelia miyamoto）感染。248 I中有1例检出宫本氏菌。感染率为0.4%。感染了B. miyamotoi是在靠近保加利亚边境的土耳其欧洲一侧的Kirklareli省的隆戈斯收集的。感染蜱的16 SrDNA、flaB和IGS序列与B.在I.欧洲的蓖麻毒素。
The hard tick-borne relapsing fever agent,Borrelia miyamotoiinfection inIxodes ricinusticks sampled from Istanbul and the countryside of Kirklareli in northwestern Turkey, was examined by TaqMan-PCR targeting 16S rDNA, nested PCR targeting 16S rDNA, the flagellin gene (flaB), and the 16S and 23S rDNA intergenic spacer (IGS), and sequencing analyses of these amplicons.B. miyamotoiwas detected in 1 out of 248I. ricinusticks (infection rate 0.4%). The tick infected withB. miyamotoiwas collected in Longos, Kirklareli province on the European side of Turkey near the Bulgarian border. The 16S rDNA,flaB, and IGS sequences from the infected tick showed high similarities to those ofB. miyamotoidetected inI. ricinusin Europe.