Immunofluorescence on pronase-digested paraffin sections: A valuable salvage technique for renal biopsies
Immunofluorescence on pronase-digested paraffin sections: A valuable salvage technique for renal biopsies
复制标题
DOI：
10.1038/sj.ki.5001990
复制
发表时间：
2006-12-01
影响因子：
19.6
通讯作者：
D'Agati, V. D.
中科院分区：
文献类型：
--
作者：
Nasr, S. H.;Galgano, S. J.;D'Agati, V. D.
冷冻组织的直接免疫荧光（IF）是医学肾脏疾病研究的首选方法。在没有肾小球的情况下，用蛋白酶提取抗原后，可以对福尔马林固定的石蜡包埋组织进行IF。在本研究中，比较了12种主要肾脏疾病的71例肾脏活检组织中冷冻组织（IF- f）和脱脂血酶处理组织（IF- p）的IF结果。应用IF-P对100%的狼疮性肾炎、急性感染后肾小球肾炎、冷球蛋白血症肾小球肾炎、原纤维性肾小球肾炎、原发性淀粉样变性、骨髓瘤型肾病、轻链范可尼综合征（LCFS）、88%的免疫球蛋白（Ig）A肾病、80%的轻链沉积病、60%的1型膜增生性肾小球肾炎、50%的特发性膜性肾小球病（MGN）和20%的抗肾小球基底膜病（GBM）。在所有疾病类别中，IF-P检测C3的敏感性低于IF-F，在MGN和抗gbm疾病中，IF-P检测IgG的敏感性低于IF-F。诊断kappa轻链染色在100%的LCFS病例中被IF-P证实，而在40%的LCFS病例中被IF-F证实。我们的结论是，对于缺乏足够皮层样本的肾活检，IF-P是一种有价值的补救性免疫组织化学技术，并且在诊断LCFS方面优于IF-F。
Direct immunofluorescence ( IF) on frozen tissue is the method of choice for the study of medical renal diseases. When no glomeruli are available, IF can be performed on the formalin-fixed paraffin-embedded tissue allocated for light microscopy after antigen retrieval with proteases. In this study, the results of IF on frozen tissue (IF-F) and on deparaffinized, pronase-treated tissue (IF-P) were compared in 71 renal biopsies representing 12 major renal diseases. Using IF-P, diagnostic findings were obtained in 100% of cases of lupus nephritis, acute post-infectious glomerulonephritis, cryoglobulinemic glomerulonephritis, fibrillary glomerulonephritis, primary amyloidosis, myeloma cast nephropathy, and light-chain Fanconi syndrome (LCFS), 88% of cases of immunoglobulin (Ig)A nephropathy, 80% of cases of light-chain deposition disease, 60% of cases of membranoproliferative glomerulonephritis type 1, 50% of cases of idiopathic membranous glomerulopathy (MGN) and 20% of cases of anti-glomerular basement membrane (GBM) disease. IF-P was less sensitive than IF-F for the detection of C3 in all disease categories and for the detection of IgG in cases of MGN and anti-GBM disease. The diagnostic kappa light-chain staining was demonstrated in 100% of cases of LCFS by IF-P versus 40% by IF-F. We conclude that IF-P is a valuable salvage immunohistochemical technique for renal biopsies lacking adequate cortical sampling for IF-F, and is superior to IF-F for the diagnosis of LCFS.