IRRADIATION WITH HE-NE-LASER INCREASES ATP LEVEL IN CELLS CULTIVATED IN-VITRO
IRRADIATION WITH HE-NE-LASER INCREASES ATP LEVEL IN CELLS CULTIVATED IN-VITRO
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DOI：
10.1016/1011-1344(94)07078-3
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发表时间：
1995-03-01
影响因子：
5.4
通讯作者：
KALENDO, G
中科院分区：
文献类型：
--
作者：
KARU, T;PYATIBRAT, L;KALENDO, G
用He-Ne激光（632.8 nm，100 J m（-2），10 s）照射单层HeLa细胞，用荧光素酶生物发光法测定照射后不同时间（5-45 min）细胞内三磷酸腺苷（ATP）的含量。照射后15 min内，培养细胞对数期ATP含量保持在对照水平（0.79 ± 0.09 × 10 ~（-15）mol/细胞），然后迅速增加，照射后20 min达到最大值（170.8%），然后缓慢下降至对照水平。发现单色红光诱导细胞ATP水平增加的能力取决于培养物的生长阶段，在培养细胞的滞后期不显著，在培养细胞的对数期增加，在对数晚期和早期平台期细胞中达到最大值（约190%）。
A monolayer of HeLa cells was irradiated with an He-Ne laser (632.8 nm, 100 J m(-2), 10 s) and the amount of adenosine triphosphate (ATP) was measured by the luceferin-luciferase bioluminescent assay technique at different times (5-45 min) after irradiation. The amount of ATP in the log phase of cultured cells remained at the control level ((0.79+/-0.09)x10(-15) mol per cell) during the first 15 min after irradiation; it then increased sharply and, after reaching a maximum (170.8%) 20 min after irradiation, decreased slowly to the control level. The ability of monochromatic red light to induce an increase in the cellular ATP level was found to depend on the growth phase of the culture, being insignificant in the lag phase of cultured cells, increasing in the log phase of cultured cells and reaching a maximum (about 190%) in cells at the late logarithmic and early plateau phase.