In Vivo Monitoring of Nitric Oxide in Tobacco Leaves by a Poly Ni(II)-Tetrasulfonated Phthalocyanine/Poly(o-phenylenediamine)/Nafion Modified Graphite Electrode
In Vivo Monitoring of Nitric Oxide in Tobacco Leaves by a Poly Ni(II)-Tetrasulfonated Phthalocyanine/Poly(o-phenylenediamine)/Nafion Modified Graphite Electrode
复制标题
利用聚镍(II)-四磺化酞菁/聚邻苯二胺/Nafion 改性石墨电极体内监测烟叶中的一氧化氮
DOI：
10.1166/nnl.2013.1568
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发表时间：
2013-06-01
影响因子：
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通讯作者：
Zhao, Yuan-Di
中科院分区：
文献类型：
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作者：
Chen, Wei;Yang, Qin;Zhao, Yuan-Di
一氧化氮参与植物的多种生理过程，但由于缺乏活体检测手段，限制了对其在植物体内功能的了解。本文构建了聚四磺酸镍酞菁/聚邻苯二胺/Nafion修饰石墨电极，并采用循环伏安法对其进行了表征。该修饰电极在磷酸盐缓冲溶液（1.18 nA/μ M）和10%烟叶匀浆（1.09 nA/μ M）中均表现出良好的灵敏度，由于聚邻苯二胺（PPD）和Nafion涂层的结合，该修饰电极对抗坏血酸（AA）、亚硝酸钠（NaNO 2）和过氧化氢（H2 O2）的干扰具有高选择性。利用该传感器对以紫外C辐射为胁迫源的烟草叶片进行了NO的连续活体监测。该传感器为植物体内NO的研究提供了一种新的途径。
Nitric Oxide participates in various physiological processes and the lack of in vivo measurement tools limits the understanding of its function in plant. In this paper, a poly Ni(II)-tetrasulfonated phthalocyanine/poly(o-phenylenediamine)/Nafion modified graphite electrode for NO detection was constructed and characterized by cyclic voltammetry. This modified electrode showed good sensitivity in both phosphate buffer saline (PBS) solution (1.18 nA/mu M) and 10% tobacco leaves homogenate (1.09 nA/mu M) with a high selectivity against the interferences from ascorbic acid (AA), sodium nitrite (NaNO2) and hydrogen peroxide (H2O2) due to the combination of poly(o-phenylenediamine) (PPD) and Nafion coatings. The continuous in vivo monitoring of NO in plant was accomplished using this NO sensor by amperometry on the tobacco leaves with ultraviolet C radiation as stressor. This NO sensor shows a great potential to facilitate the NO research in plant.