Treatment with Astragali Radix and Angelicae Radix Enhances Erythropoietin Gene Expression in the Cyclophosphamide-Induced Anemic Rat
Treatment with Astragali Radix and Angelicae Radix Enhances Erythropoietin Gene Expression in the Cyclophosphamide-Induced Anemic Rat
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DOI：
10.1089/jmf.2007.0727
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发表时间：
2009-06-01
影响因子：
2.4
通讯作者：
Park, Seong Kyu
中科院分区：
文献类型：
--
作者：
Chang, Mun Seog;Kim, Do Rim;Park, Seong Kyu
贫血是发病率和疾病的常见原因，并降低生活质量。本研究探讨黄芪（AMW）和当归（AGW）联合治疗（AAC）对环磷酰胺（CYP）诱导的贫血大鼠红细胞生成素（EPO）表达和血液学指标的影响。将4周龄雄性Sprague-Dawley大鼠按实验方案分为有或无cypp诱导贫血、单用或联合用药5组。治疗后采用逆转录-聚合酶链反应评价AAC对促红细胞生成素表达的影响，并测定血液及血清学参数。与正常组相比，cypp处理组EPO mRNA水平较低，而aac处理组EPO mRNA水平较高。cypp治疗组血清铁浓度、总铁结合能力和维生素B-12水平较低，而aac治疗组正常或接近正常。cypp治疗组体重增加少于正常组，但AAC组体重增加部分正常化。饲料效率以CYP组最低，但差异不显著。测定红细胞、白细胞、血小板数量、红细胞比容和血红蛋白水平。结果显示，与cypp处理组和AAC处理组相比，cypp处理组血细胞数量减少，而AAC-、AMW-和agw处理组血细胞数量显著增加。AAC可增强cypp诱导的贫血大鼠EPO mRNA表达，改善血液学指标和维生素B-12状态。
Anemia is a common cause of morbidity and disease and reduces the quality of life. This study examined the effect of a combination treatment (AAC) using Astragali radix (AMW) and Angelicae radix (AGW) in cyclophosphamide (CYP)-induced anemic rats on erythropoietin (EPO) expression and hematological parameters. Male 4-week-old Sprague-Dawley rats were divided into five groups with or without CYP-induced anemia and individual or the combined herbal treatments according to the experimental protocol. After treatment, reverse transcription-polymerase chain reaction was used to evaluate the effects of AAC on erythropoietin expression, and blood and serological parameters were measured. The EPO mRNA levels were lower in the CYP-treated group, compared to the normal group, and higher in the AAC-treated group. In the CYP-treated group, the serum iron concentration, total iron-binding capacity, and vitamin B-12 level were lower, but these were normal or almost normal in the AAC-treated group. The CYP-treated group gained less weight than the normal group, but weight gain was partially normalized in the AAC group. The feed efficiency ratio was lowest in the CYP group, but the differences were not significant. The numbers of red blood cells, white blood cells, and platelets, the hematocrit, and the hemoglobin level were measured. The results revealed a reduced number of blood cells in the CYP-treated group, whereas the AAC-, AMW-, and AGW-treated groups showed significantly enhanced blood cell numbers compared to the CYP-treated control group and the AAC- treated group. AAC enhanced EPO mRNA expression in the CYP-induced anemic rat and improved the hematological parameters and vitamin B-12 status.