Galectin-3 Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Reduces Amyloid-Beta42 Neurotoxicity In Vitro
Galectin-3 Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Reduces Amyloid-Beta42 Neurotoxicity In Vitro
复制标题
人脐带血间充质干细胞分泌的半乳糖凝集素 3 可降低体外淀粉样蛋白 - Beta42 神经毒性
DOI：
10.1016/j.bpj.2010.12.2460
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发表时间：
2011
影响因子：
3.4
通讯作者：
J. Chang
中科院分区：
文献类型：
--
作者：
Ju;Dong Hyun Kim;Dal;J. Kim;S. Jeong;H. Jeon;E. Lee;Yoon;W. Oh;J. Chang
李根镇，禹振锡，李恩慧。在骨骼肌中，基质相互作用分子1 （STIM1）是肌浆网的钙传感器，而Orai1是细胞外Ca2+进入通道。已知这两种蛋白质是骨骼肌储存操作Ca2+进入（SOCE）的组成部分。在这里，我们检测了骨骼肌细胞分化过程中STIM1和Orai1的表达。在小鼠原代骨骼肌母细胞向肌管分化的过程中，Orai1的表达逐渐增加，直到分化第2天，并在分化后的第2天保持一定水平。分化过程中STIM1表达增加。用抗STIM1和抗Orai1抗体对成肌细胞和肌管进行免疫细胞化学分析，结果表明，在分化过程中，除细胞核部分外，STIM1和Orai1在细胞内均有共定位。这些结果表明，STIM1和Orai1介导的SOCE在骨骼肌分化中发挥了一定的作用。
Keon Jin Lee, Jin Seok Woo, Eun Hui Lee. In skeletal msucle, stromal interaction molecule 1 (STIM1) is the calcium sensor of sarcoplasmic reticulum and Orai1 is an extracellular Ca2+-entry channel. These two proteins are known to be components of store-operated Ca2+-entry (SOCE) in skeletal muscle. Here, we examined expression of STIM1 and Orai1 during skeletal muscle cell differentiation. During differentiation of primary mouse skeletal myoblasts to myotubes, Orai1 expression was gradually increased until differentiation day 2 and kept at a certain level on further days of differentiation. STIM1 expression was increased during differentiation. Immunocytochemistry of myoblasts and myotubes with anti-STIM1 and anti-Orai1 antibodies showed that STIM1 and Orai1 were colocalized all over the cells except for nucleus parts during differentiation. These results suggest that SOCE by STIM1 and Orai1 plays a certain role in skeletal muscle differentiation.