Group 1 CD1 genes in rabbit
Group 1 CD1 genes in rabbit
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DOI：
10.4049/jimmunol.166.1.403
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发表时间：
2001-01-01
影响因子：
4.4
通讯作者：
Knight, KL
中科院分区：
文献类型：
--
作者：
Hayes, SM;Knight, KL
CD1 是一种 Ag 呈递分子，可以向 T 细胞呈递脂质和糖脂。CD1 基因首先在人类中发现，从那时起，在迄今为止检查的每个哺乳动物物种中都发现了同源物。十多年前，CD1B 和 CD1D 同源物在兔子体内被发现。我们通过鉴定额外的 CD1 基因扩展了这项早期研究，目标是开发兔子作为研究 CD1 蛋白功能的动物模型。我们构建了胸腺细胞 cDNA 文库，并用 CD1 特异性探针筛选该文库。基于对从文库中获得的CD1(+) cDNA克隆的核苷酸序列分析，我们鉴定了两个CD1A基因和一个CD1E基因，并确定了先前鉴定的CD1B基因的完整序列。 CD1E(+) cDNA 克隆缺乏跨膜和细胞质结构域，如果翻译，将编码可溶性或分泌性 CD1E 蛋白。此外，表达研究表明 CD1 基因在外周淋巴组织以及皮肤、肠道和肺中表达。有趣的是，兔 B 细胞群表达了 CD1A2、CD1B 和 CD1E 基因。兔子具有由至少两个 CD1A 基因、一个 CD1B 基因、一个 CD1D 基因和一个 CD1E 基因组成的复杂 CD1 基因座，是研究 CD1 蛋白的动物模型的绝佳候选者。
CD1 is an Ag-presenting molecule that can present lipids and glycolipids to T cells, The CD1 genes were first identified in the human, and since then, homologs have been identified in every mammalian species examined to date. Over a decade ago, CD1B and CD1D homologs were identified in the rabbit. We have extended this earlier study by identifying additional CD1 genes with the goal of developing the rabbit as an animal model to study the function of CD1 proteins. We constructed a thymocyte cDNA library and screened the library with CD1-specific probes. Based on nucleotide sequence analyses of the CD1(+) cDNA clones obtained from the library, we have identified two CD1A genes and one CD1E gene as well as determined the complete sequence of the previously identified CD1B gene. The CD1E(+) cDNA clones lacked the transmembrane and cytoplasmic domains and, if translated, would encode for a soluble or secreted CD1E protein, In addition, expression studies demonstrated that the CD1 genes were expressed in peripheral lymphoid tissues as well as in skin, gut, and lung. Of interest is the finding that CD1A2, CD1B, and CD1E genes were found to be expressed by rabbit B cell populations. The rabbit, with a complex CD1 locus composed of at least two CD1A genes, one CD1B gene, one CD1D gene, and one CD1E gene, is an excellent candidate as an animal model to study CD1 proteins.