Experimental production of intercellular antibodies in monkeys.
Experimental production of intercellular antibodies in monkeys.
复制标题
猴子细胞间抗体的实验生产。
DOI：
10.1111/j.1749-6632.1983.tb22222.x
复制
发表时间：
1983
影响因子：
5.2
通讯作者：
Beutner,EH
中科院分区：
文献类型：
--
作者：
Krasny,SA;Beutner,EH
为了诱导天疱疮抗体的形成，一些研究小组进行了兔免疫实验。通过间接免疫荧光（IF）检测到，这些药物诱导抗体进入分层上皮细胞间区。然而，这些都没有在体内证明结合的细胞间抗体，因此表明产生的抗体是天疱疮样的。这些抗体产生类似于天疱疮抗体的间接IF染色模式。从抗体生产者的角度来看。最重要的区别是，真正的天疱疮抗体在体内与正常皮肤和粘膜中的抗原结合并产生上皮损伤，而天疱疮样抗体则不会。Michel， Schiltz和他们的同事~~~~关于天疱疮抗体与皮肤外体结合并在缺乏补体的情况下发生上皮内病变的报告是天疱疮抗体致病性潜力的最令人信服的实验证据。最近，我们已经将这种器官培养系统用于粘膜外植体。本研究的目的是用含天疱疮抗原的人食管黏膜盐水提取物免疫猴子，以诱导对分层上皮细胞间抗原的抗体。我们还描述了这些抗体在器官培养中的致病潜力。因为许多报告描述了由d -青霉胺引起的人类天疱疮病例，一些动物用这种药物进行了预处理
Efforts to induce the formation of pemphigus antibodies have led several research groups to conduct rabbit immunization experiments. These have induced antibodies to the intercellular areas of stratified epithelia as detected by the indirect immunofiuorescence (IF) te~ t.'.~.'.''However, none of these demonstrated in vivo bound intercellular antibodies, thus suggesting that antibodies produced were pemphigus-like. These antibodies yield indirect IF staining patterns resembling those of pemphigus antibodies. From the standpoint of the antibody producer. the most important difference is that true pemphigus antibodies bind in vivo to their antigens in the normal skin and mucosa and produce epithelial damage whereas pemphigus-like antibodies fail to doThe reports of Michel, Schiltz, and their co-worker~~~~ on the binding of pernphigus antibodies to skin explants with subsequent development of intraepithelial lesions in the absence of complement are the most convincing experimental evidence for the pathogenic potential of pemphigus antibodies. Recently, we have adapted this organ culture system for use with mucosal explants. The purpose of this study was to elicit antibodies to the intercellular antigens of stratified epithelia by immunization of monkeys with a saline extract of human esophageal mucosa containing pemphigus antigen. We also characterized these antibodies with regard to their pathogenic potential in organ culture. Because a number of reports describe cases of human pemphigus provoked by D-penicillamine, some of the animals were pretreated with this drug.'.'*