Transcriptome analysis reveals unique C4-like photosynthesis and oil body formation in an arachidonic acid-rich microalga Myrmecia incisa Reisigl H4301.
Transcriptome analysis reveals unique C4-like photosynthesis and oil body formation in an arachidonic acid-rich microalga Myrmecia incisa Reisigl H4301.
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转录组分析揭示了富含花生四烯酸的微藻 Myrmecia incisa Reisigl H4301 独特的类 C4 光合作用和油体形成。
DOI：
10.1186/1471-2164-14-396
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发表时间：
2013-06-13
期刊：
影响因子：
4.4
通讯作者：
Zhou ZG
中科院分区：
文献类型：
--
作者：
Ouyang LL;Chen SH;Li Y;Zhou ZG
花生四烯酸（ArA）是哺乳动物脑磷脂的主要成分之一，对人类健康具有重要意义。对ArA的兴趣激发了对新的可持续来源的寻找，并且由于细胞内ArA的高含量，绿色微生物Myrmincisa Reisigl H4301已被发现是潜在的ArA生产者。为了获得更多关于代谢途径的分子信息，包括在非模式微生物中ArA的生物合成，进行转录组学分析。454个焦磷酸测序产生了371，740个高质量读段，这些读段被组装成51，908个独特序列，由22，749个重叠群和29，159个单例组成。通过BLAST分析共注释了11，873个独特序列，其中3，733个被分配到基因本体论（GO）类别。京都基因和基因组百科全书（KEGG）途径分析揭示了M.切口详细分析了油脂的生物合成途径，特别是ArA和三酰甘油（TAG）的生物合成途径，认为TAG是在钙曙红或油球相关蛋白的作用下积累在胞质油体中的。此外，类胡萝卜素的生物合成途径进行了讨论。本研究对M. incisa使我们对光合作用、ArA的从头生物合成、类胡萝卜素的代谢和TAG在M.切口这些发现为研究和经济开发这种富含ArA的微生物提供了分子基础。
Arachidonic acid (ArA) is important for human health because it is one of the major components of mammalian brain membrane phospholipids. The interest in ArA inspired the search for a new sustainable source, and the green microalga Myrmecia incisa Reisigl H4301 has been found a potential ArA-producer due to a high content of intracellular ArA. To gain more molecular information about metabolism pathways, including the biosynthesis of ArA in the non-model microalga, a transcriptomic analysis was performed. The 454 pyrosequencing generated 371,740 high-quality reads, which were assembled into 51,908 unique sequences consisting of 22,749 contigs and 29,159 singletons. A total of 11,873 unique sequences were annotated through BLAST analysis, and 3,733 were assigned to Gene Ontology (GO) categories. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis uncovered a C4-like photosynthesis pathway in M. incisa. The biosynthesis pathways of lipid particularly those of ArA and triacylglycerol (TAG) were analyzed in detail, and TAG was proposed to be accumulated in oil bodies in the cytosol with the help of caleosin or oil globule-associated proteins. In addition, the carotenoid biosynthesis pathways are discussed. This transcriptomic analysis of M. incisa enabled a global understanding of mechanisms involved in photosynthesis, de novo biosynthesis of ArA, metabolism of carotenoids, and accumulation of TAG in M. incisa. These findings provided a molecular basis for the research and possibly economic exploitation of this ArA-rich microalga.