Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology.
Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology.
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DOI：
10.3389/fpls.2021.697318
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发表时间：
2021
影响因子：
5.6
通讯作者：
Sathishkumar R
中科院分区：
文献类型：
--
作者：
Bharadwaj R;Kumar SR;Sharma A;Sathishkumar R
植物是高价值特化代谢物的重要来源，具有重要的生理和生态功能。负责合成特殊代谢物的基因通常聚集在一起协调表达，这在细菌和丝状真菌中很常见。与原核生物的基因聚类类似，植物也有编码特殊代谢物生物合成酶的基因簇。在整个植物界中，已经确定了20多个参与多种代谢物生物合成的基因簇。近年来的研究表明，基因集群是通过基因复制和初级代谢途径基因的新功能化而进化的。通常，这些簇在核小体水平上受到严格调控。与特殊代谢物相关的基因簇的流行为高度有用的生物分子的未开发来源提供了一个有吸引力的可能性。因此，需要对植物中新的生物合成途径进行鉴定和功能表征。在这篇综述中，我们总结了对基因簇进化的见解，并讨论了特定基因簇在特定代谢物生物合成中的组织和重要性。在一些重要的基因簇中运作的调控机制也被简要描述。最后，我们强调了基因簇在开发未来代谢工程或合成生物学策略以异源生产新型代谢物方面的重要性。
Plants are a remarkable source of high-value specialized metabolites having significant physiological and ecological functions. Genes responsible for synthesizing specialized metabolites are often clustered together for a coordinated expression, which is commonly observed in bacteria and filamentous fungi. Similar to prokaryotic gene clustering, plants do have gene clusters encoding enzymes involved in the biosynthesis of specialized metabolites. More than 20 gene clusters involved in the biosynthesis of diverse metabolites have been identified across the plant kingdom. Recent studies demonstrate that gene clusters are evolved through gene duplications and neofunctionalization of primary metabolic pathway genes. Often, these clusters are tightly regulated at nucleosome level. The prevalence of gene clusters related to specialized metabolites offers an attractive possibility of an untapped source of highly useful biomolecules. Accordingly, the identification and functional characterization of novel biosynthetic pathways in plants need to be worked out. In this review, we summarize insights into the evolution of gene clusters and discuss the organization and importance of specific gene clusters in the biosynthesis of specialized metabolites. Regulatory mechanisms which operate in some of the important gene clusters have also been briefly described. Finally, we highlight the importance of gene clusters to develop future metabolic engineering or synthetic biology strategies for the heterologous production of novel metabolites.
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影响因子：
56.9
作者：
Frey, M;Chomet, P;Gierl, A
通讯作者：
Gierl, A
影响因子：
14.8
作者：
Bok, Jin Woo;Chiang, Yi-Ming;Szewczyk, Edyta;Reyes-Domingez, Yazmid;Davidson, Ashley D.;Sanchez, James F.;Lo, Hsien-Chun;Watanabe, Kenji;Strauss, Joseph;Oakley, Berl R.;Wang, Clay C. C.;Keller, Nancy P.
通讯作者：
Keller, Nancy P.
影响因子：
4.2
作者：
Braun SA;Baran J;Schrumpf H;Buhren BA;Bölke E;Homey B;Gerber PA
通讯作者：
Gerber PA
DOI：
10.1007/bf00280297
发表时间：
1991-12-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
CABALLERO, JL;MARTINEZ, E;HOPWOOD, DA
通讯作者：
HOPWOOD, DA
影响因子：
4.6
作者：
Barchi, Lorenzo;Pietrella, Marco;Rotino, Giuseppe Leonardo
通讯作者：
Rotino, Giuseppe Leonardo