Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae.
Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae.
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DOI：
10.1385/1-59259-750-5:001
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Buratowski, Stephen
中科院分区：
文献类型：
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作者：
Keogh, Michael-Christopher;Buratowski, Stephen
染色质免疫沉淀(ChIP)技术已经被用来确定DNA结合蛋白在哪里以及在什么条件下与特定的DNA序列相关联。蛋白质在体内与甲醛交联，然后分离并剪切染色质。然后免疫沉淀目的蛋白，并通过聚合酶链式反应鉴定相关的DNA序列。虽然这项技术最初是设计用来检测DNA结合蛋白的，但它也可以用来监测与转录复合体相关的mRNA处理因子。
The chromatin immunoprecipitation (ChIP) technique has been used to determine where and under what conditions DNA binding proteins associate with specific DNA sequences. Proteins are crosslinked in vivo with formaldehyde, and chromatin is then isolated and sheared. The protein of interest is then immunoprecipitated and the associated DNA sequences identified via PCR. Although this technique was originally designed to assay DNA binding proteins, it can also be used to monitor mRNA processing factors associated with transcription complexes.