Uptake of glycine, GABA and glutamate by synaptic vesicles isolated from different regions of rat CNS
Uptake of glycine, GABA and glutamate by synaptic vesicles isolated from different regions of rat CNS
复制标题
大鼠中枢神经系统不同区域分离的突触小泡对甘氨酸、GABA 和谷氨酸的摄取
DOI：
10.1016/0304-3940(91)90465-6
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发表时间：
1991
影响因子：
2.5
通讯作者：
F. Fonnum
中科院分区：
文献类型：
--
作者：
H. Christensen;F. Fonnum
通过密度梯度离心从大脑皮层、皮质下端脑、全脑和脊髓中分离突触囊泡组分。比较了Mg ~（2+）-ATP依赖的囊泡摄取和Na ~+-依赖的突触体对甘氨酸、GABA和谷氨酸的摄取。所有这些区域都含有GABA作为抑制性神经递质，而甘氨酸仅在脊髓中起主导作用。GABA和甘氨酸的摄取在不同的囊泡组分之间的比例是相似的，和突触体摄取的比例有很大的不同。与此相反，谷氨酸和GABA的摄取在不同区域的囊泡之间的比例不同，这些比例对应的突触体摄取的比例。这些结果表明，甘氨酸从非甘氨酸末端进入突触囊泡，我们认为，GABA和甘氨酸可以进入相同的囊泡人口。
Synaptic vesicle fractions have been isolated from cerebral cortex, subcortical telencephalon, whole brain and spinal cord by density gradient centrifugation. The Mg2+ATP-dependent vesicular uptake and the Na+-dependent synaptosomal uptake of glycine, GABA andl-glutamate has been compared in the different regions. All these regions contain GABA as inhibitory neurotransmitter, whereas glycine only plays a dominant role as such in the spinal cord. The ratio between GABA and glycine uptake in the different vesicle fractions was similar, and the ratios differed greatly from the ratios in the synaptosomal uptake. In contrast, the ratio between glutamate and GABA uptake in vesicles from different regions differed, and these ratios corresponded to the ratios in the synaptosomal uptake. These results indicate that glycine is taken up into synaptic vesicles from non-glycine terminals, and we suggest that GABA and glycine can be taken up into the same vesicle population.